Title:
METHOD FOR DETECTING HUMAN PAPILLOMAVIRUS
Document Type and Number:
WIPO Patent Application WO/2020/050350
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for detecting HPV with high sensitivity in a subject, and particularly to provide a method for detecting HPV in semen or urine. A method for amplifying HPV in a subject by PCR reaction and detecting HPV infection, the method for detecting HPV infection including: (a) in a first PCR, performing amplification through use of an adapter-primer pair in which an adapter comprising a sequence which does not anneal with the HPV genome, a human genome, and the genome of a virus or microbe that infects a human is appended to a primer for hybridizing specifically with an HPV gene; and, (b) in a second PCR, amplifying the amplification product of (a) through use of a primer pair comprising a sequence that is complementary to the adapter.
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Inventors:
TAKIMOTO YOSUKE (JP)
HAGIWARA KEITARO (JP)
KANAI MASATAKE (JP)
HAGIWARA KEITARO (JP)
KANAI MASATAKE (JP)
Application Number:
PCT/JP2019/034908
Publication Date:
March 12, 2020
Filing Date:
September 05, 2019
Export Citation:
Assignee:
HEALTHCARE SYSTEMS CO LTD (JP)
DANTTE INC (JP)
DANTTE INC (JP)
International Classes:
C12Q1/6888; C12Q1/6855; C12Q1/686; C12N15/09
Domestic Patent References:
| WO2007047572A2 | 2007-04-26 | |||
| WO1996015139A1 | 1996-05-23 | |||
| WO2008118839A1 | 2008-10-02 |
Foreign References:
| CN102399863A | 2012-04-04 | |||
| JP2009515545A | 2009-04-16 | |||
| JP2000500342A | 2000-01-18 | |||
| JP2018102284A | 2018-07-05 | |||
| CN104560962A | 2015-04-29 | |||
| JP2009528051A | 2009-08-06 | |||
| CN103409560A | 2013-11-27 |
Other References:
RUGHOOPUTH, SANJIV ET AL.: "Detection of human papillomavirus from archival tissues in cervical cancer patients in Mauritius", J. CLIN. VIROL., vol. 35, 2006, pages 173 - 178, XP028037741, DOI: 10.1016/j.jcv.2005.03.010
MORI, SEIICHIRO ET AL.: "Biased amplification of human papillomavirus DNA in specimens containing multiple human papillomavirus types by PCR with consensus primers", CANCER SCI., vol. 102, 2011, pages 1223 - 1227, XP055689652
RONAI, ZE'EV ET AL.: "Quantitative enriched PCR (QEPCR), a highly sensitive method for detection of K-ras oncogene mutation", HUM. MUTAT., vol. 10, 1997, pages 322 - 325, XP009028801, DOI: 10.1002/(SICI)1098-1004(1997)10:4<322::AID-HUMU9>3.0.CO;2-I
MORI, SEIICHIRO ET AL.: "Biased amplification of human papillomavirus DNA in specimens containing multiple human papillomavirus types by PCR with consensus primers", CANCER SCI., vol. 102, 2011, pages 1223 - 1227, XP055689652
RONAI, ZE'EV ET AL.: "Quantitative enriched PCR (QEPCR), a highly sensitive method for detection of K-ras oncogene mutation", HUM. MUTAT., vol. 10, 1997, pages 322 - 325, XP009028801, DOI: 10.1002/(SICI)1098-1004(1997)10:4<322::AID-HUMU9>3.0.CO;2-I
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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