Title:
METHOD FOR DETECTING MICRORNA IN BIOLOGICAL SAMPLE, COMPOSITION OF HYDROCHLORIDE OF NEUTRAL AMINO ACID, AND CONTAINER
Document Type and Number:
WIPO Patent Application WO/2022/209943
Kind Code:
A1
Abstract:
The detection level decreases as storage time lengthens after collection of a biological sample containing microRNA. In addition, a relatively long time is required to dissolve a hydrochloride of a neutral amino acid, which is crystalline, in water. A hydrochloride of a neutral amino acid is an acidic substance and irritating to the skin and mucous membranes and therefore requires care in handling in terms of safety. The present invention is a method for detecting microRNA in a biological sample, characterized by including a step for mixing an acid with a biological sample, a step for storage for a predetermined length of time, a step for recovering the microRNA in the biological sample, and a detection step. The present invention also provides a homogenous semisolid composition comprising a hydrochloride of a neutral amino acid, water, and an alcohol.
Inventors:
KOSHI YOICHIRO (JP)
WAKAO OSAMU (JP)
WAKAO OSAMU (JP)
Application Number:
PCT/JP2022/012206
Publication Date:
October 06, 2022
Filing Date:
March 17, 2022
Export Citation:
Assignee:
TORAY INDUSTRIES (JP)
International Classes:
C12Q1/6806; C12M1/00; C12N15/09; C12N15/10; C12Q1/6837
Domestic Patent References:
| WO2021122846A1 | 2021-06-24 | |||
| WO2016148073A1 | 2016-09-22 |
Foreign References:
| JP2017520268A | 2017-07-27 | |||
| JPH08508637A | 1996-09-17 | |||
| JP2018523478A | 2018-08-23 | |||
| JP2018504915A | 2018-02-22 | |||
| JP2011517283A | 2011-06-02 | |||
| JPH08508637A | 1996-09-17 |
Other References:
TAKETOSHI HATA; KOSUKE MURAKAMI; HAJIME NAKATANI; YASUNARI YAMAMOTO; TSUKASA MATSUDA; NAOHITO AOKI: "Isolation of bovine milk- derived microvesicles carrying mRNAs and microRNAs.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 396, no. 2, 28 May 2010 (2010-05-28), pages 528 - 533, XP002716646, DOI: 10.1016/j.bbrc.2010.04.135
JOURNAL OF APPLIED POLYMER SCIENCE, vol. 123, 2011, pages 3772 - 3780
JOURNAL OF APPLIED POLYMER SCIENCE, vol. 123, 2011, pages 3772 - 3780
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