Title:
METHOD FOR DETECTING MULTIPLE TARGET NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO/2023/125562
Kind Code:
A1
Abstract:
The present invention relates to the field of molecular biology, in particular to a method for detecting multiple target nucleic acids in a single sample container. The present invention particularly relates to a method for multiplex detection based on digital PCR. The method comprises: mixing a primer probe composition, a sample to be tested and an amplification reagent to obtain a reaction system; placing the reaction system in a condition that enables a nucleic acid polymerase to perform hybridization and extension reaction, so as to obtain a reaction product; and performing signal acquisition on the reaction product. The described method can realize detection of multiple target nucleic acids, is simple to operate and can save time.
Inventors:
ZHAO YUHANG (CN)
HU WANER (CN)
WANG HAIXIN (CN)
HUANG QIUPING (CN)
HU WANER (CN)
WANG HAIXIN (CN)
HUANG QIUPING (CN)
Application Number:
PCT/CN2022/142473
Publication Date:
July 06, 2023
Filing Date:
December 27, 2022
Export Citation:
Assignee:
MACCURA BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12M1/34; C12Q1/686; C12M1/38; C12N15/11
Domestic Patent References:
| WO2010013017A1 | 2010-02-04 |
Foreign References:
| CN112111566A | 2020-12-22 | |||
| CN113699033A | 2021-11-26 | |||
| CN103276094A | 2013-09-04 | |||
| US20130045881A1 | 2013-02-21 | |||
| US20140315747A1 | 2014-10-23 |
Other References:
YIQUN LIAO, WANG XIAOBO, SHA CHAO, XIA ZHONGMIN, HUANG QIUYING, LI QINGGE: "Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, GB, vol. 41, no. 7, 1 April 2013 (2013-04-01), GB , pages 1 - 11, XP055558092, ISSN: 0305-1048, DOI: 10.1093/nar/gkt004
Attorney, Agent or Firm:
YUHONG INTELLECTUAL PROPERTY LAW FIRM (CN)
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