Title:
METHOD FOR DETECTING NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO/2010/130877
Kind Code:
A3
Abstract:
This invention relates to detecting nucleic acids. It employs a double-stranded oligonucleotide probe comprising i)a first probe comprising a first label moiety capable of emitting a measurable signal, and ii) a second probe being partially complementary with the first probe and comprising a second label moiety capable of interacting with the first moiety when brought in close proximity with each other, the second moiety being a quencher or acceptor of emission of the first moiety. The first or second probe comprises a sequence being complementary to that of a target nucleotide, and the second or first probe, respectively, comprises a sequence being complementary to a complement of the target nucleotide sequence of the nucleic acid to be detected. The first and the second moieties are attached to the first and second probes respectively in a manner wherein the distance between the first and second moieties is not more than 7 base pairs apart. The complementary sequences of the double-stranded probe is shorter than the full sequence of both the first and second single-stranded probes. The first and second probes have a higher Tm when hybridized with the target nucleotide sequence compared to the Tm of the double-stranded probe. The intensity of the signal of the first label when the first probe is not hybridized to the second probe is higher or lower than the intensity of the signal of the first label when the first probe is hybridized to the second probe. This invention also relates to oligonucleotides for determining Chlamydia trachomatis.
Inventors:
VON LODE PIIA (FI)
SYRJAELAE ANNIINA (FI)
LOEVGREN TIMO (FI)
SOUKKA TERO (FI)
SYRJAELAE ANNIINA (FI)
LOEVGREN TIMO (FI)
SOUKKA TERO (FI)
Application Number:
PCT/FI2010/050380
Publication Date:
January 27, 2011
Filing Date:
May 11, 2010
Export Citation:
Assignee:
ABACUS DIAGNOSTICA OY (FI)
VON LODE PIIA (FI)
SYRJAELAE ANNIINA (FI)
LOEVGREN TIMO (FI)
SOUKKA TERO (FI)
VON LODE PIIA (FI)
SYRJAELAE ANNIINA (FI)
LOEVGREN TIMO (FI)
SOUKKA TERO (FI)
International Classes:
C12Q1/68
Domestic Patent References:
| WO2008044129A2 | 2008-04-17 | |||
| WO2008021446A2 | 2008-02-21 | |||
| WO2008146306A2 | 2008-12-04 | |||
| WO2002030946A1 | 2002-04-18 |
Foreign References:
| CN101041864A | 2007-09-26 | |||
| EP0861906A1 | 1998-09-02 | |||
| EP1666609A1 | 2006-06-07 | |||
| EP0232967A2 | 1987-08-19 | |||
| CN101363041A | 2009-02-11 |
Other References:
DATABASE Geneseq [online] 11 December 2008 (2008-12-11), "Chlamydia trachomatis cryptic plasmid ORF1 gene sequence.", XP002592204, retrieved from EBI accession no. GSN:ATS61935 Database accession no. ATS61935
CHENG JINPING ET AL: "Real-time PCR genotyping using displacing probes.", NUCLEIC ACIDS RESEARCH 2004 LNKD- PUBMED:15087493, vol. 32, no. 7, 2004, pages E61, XP002592205, ISSN: 1362-4962
LI QINGGE ET AL: "A new class of homogeneous nucleic acid probes based on specific displacement hybridization.", NUCLEIC ACIDS RESEARCH 15 JAN 2002 LNKD- PUBMED:11788731, vol. 30, no. 2, 15 January 2002 (2002-01-15), pages E5, XP002592206, ISSN: 1362-4962
DATABASE Geneseq [online] 4 May 2000 (2000-05-04), XP002611779, retrieved from EBI accession no. GSN:AAZ88369 Database accession no. AAZ88369
See also references of EP 2430187A2
CHENG JINPING ET AL: "Real-time PCR genotyping using displacing probes.", NUCLEIC ACIDS RESEARCH 2004 LNKD- PUBMED:15087493, vol. 32, no. 7, 2004, pages E61, XP002592205, ISSN: 1362-4962
LI QINGGE ET AL: "A new class of homogeneous nucleic acid probes based on specific displacement hybridization.", NUCLEIC ACIDS RESEARCH 15 JAN 2002 LNKD- PUBMED:11788731, vol. 30, no. 2, 15 January 2002 (2002-01-15), pages E5, XP002592206, ISSN: 1362-4962
DATABASE Geneseq [online] 4 May 2000 (2000-05-04), XP002611779, retrieved from EBI accession no. GSN:AAZ88369 Database accession no. AAZ88369
See also references of EP 2430187A2
Attorney, Agent or Firm:
TURUN PATENTTITOIMISTO OY (Turku, FI)
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