METHOD FOR DETECTING OR QUANTIFYING NUCLEIC ACID

Title:

METHOD FOR DETECTING OR QUANTIFYING NUCLEIC ACID

Document Type and Number:

WIPO Patent Application WO/2021/153709

Kind Code:

A1

Abstract:

The present invention addresses the problem of providing a detection method and a quantification method enabling more sensitive detection with respect to methods for detecting a target nucleic acid by the PALSAR method.　The present invention provides a method for collecting a target nucleic acid in a sample, the method comprising the following steps. (i) Step for bringing the target nucleic acid in the sample into contact with a capture probe and an assist probe to cause hybridization. In this step, the capture probe includes a nucleic acid probe (A) and an adaptor or linker or a solid phase (B) adjacent to the nucleotide at the 3' end or the 5' end of the nucleic acid probe. The nucleic acid probe includes a first base sequence and a second base sequence complementary to the entire sequence or a partial sequence of the target nucleic acid. The assist probe includes a sequence of a 6- to 9-mer that is complementary to the entire sequence or a partial sequence of the first base sequence of the nucleic acid probe, while being able to be linked adjacent to the target nucleic acid. (ii) Step for collecting the hybridization product contained in the sample.

More Like This:

JP2004527201	Methods for Producing Transgenic Plants with Increased Alfin 1 Expression, Root Growth and Specific Gene Activation of Roots
WO/1996/034892	BISPECIFIC ANTIBODIES IN WHICH THE BINDING CAPABILITY IS REVERSIBLY INHIBITED BY A PHOTOCLEAVABLE MOIETY
JP4006381	Nucleic acid high density immobilization

Inventors:

OOMORI AKANE (JP)
OSAWA MASAKO (JP)

Application Number:

PCT/JP2021/003144

Publication Date:

August 05, 2021

Filing Date:

January 29, 2021

Export Citation:

Click for automatic bibliography generation Help

Assignee:

SEKISUI MEDICAL CO LTD (JP)

International Classes:

C12N15/09; C12N15/10; C12Q1/6813; C12Q1/6876

Domestic Patent References:

WO2013085026A1	2013-06-13
WO2013172305A1	2013-11-21

Foreign References:

JP2012070635A	2012-04-12
JP2012070635A	2012-04-12
JP2020014497A	2020-01-30

Other References:

BIOCHEM BIOPHYS RES COMMUN., vol. 467, no. 4, 27 November 2015 (2015-11-27), pages 1012 - 8
WEI XDAI GMARCUCCI GLIU ZHOYT DBLUM WCHAN KK: "A specific picomolar hybridization-based ELISA assay for the determination of phosphorothioate oligonucleotides in plasma and cellular matrices", PHARM RES, vol. 23, no. 6, June 2006 (2006-06-01), pages 1251 - 64, XP019405116, DOI: 10.1007/s11095-006-0082-3
See also references of EP 4098744A4

Attorney, Agent or Firm:

MOEGI PATENT OFFICE (JP)

Download PDF:

View/Download PDF PDF Help

Previous Patent: CHOLESTEROL ANALOG HAVING ANTI-HELICOBACTER PYLORI ACTIVITY

Next Patent: PLATINUM-GROUP METAL RECOVERY METHOD, COMPOSITION CONTAINING PLATINUM-GROUP METALS, AND CERAMIC MATE...