Title:
METHOD FOR DETECTING SIGLECS FAMILY IN CHORION, AND TEST KIT AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/174620
Kind Code:
A1
Abstract:
Disclosed by the present invention are a method for detecting the Siglecs family in a chorion, and a test kit and application thereof: preparing specific primers for Siglec 1-3, Siglec 5-12, and Siglec 14-15 in the chorion Siglecs family; coating the specific primers on a cell culture plate; mixing a sample to be tested with a reaction liquid, then adding the coated cell culture plate to perform PCR quantitative detection and using the test kit for performing the detection method; the above can be used for measuring the nucleic acid level of the Siglecs family in the chorion, and is not suitable for application in repeated natural abortion for unknown reasons, particularly in detection of repeated natural abortion caused by the failure of the embryo adhesion capability, etc. as a result of the embryo implantation capability being affected.
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Inventors:
MA FANG (CN)
FENG YING (CN)
ZHANG YUE (CN)
ZHANG LIN (CN)
MA QIANHONG (CN)
LIN YI (CN)
FENG YING (CN)
ZHANG YUE (CN)
ZHANG LIN (CN)
MA QIANHONG (CN)
LIN YI (CN)
Application Number:
PCT/CN2020/082323
Publication Date:
September 10, 2021
Filing Date:
June 04, 2020
Export Citation:
Assignee:
WES CHINA SECOND UNIV HOSPITAL SICHUAN UNIV (CN)
International Classes:
C12Q1/6883; C12Q1/6851
Domestic Patent References:
| WO2017079741A1 | 2017-05-11 |
Foreign References:
| CN106319031A | 2017-01-11 | |||
| CN102618542A | 2012-08-01 |
Other References:
SAMMAR MAREI; SIWETZ MONIKA; MEIRI HAMUTAL; FLEMING VIKTOR; ALTEVOGT PETER; HUPPERTZ BERTHOLD: "Expression of CD24 and Siglec-10 in first trimester placenta: implications for immune tolerance at the fetal–maternal interface", HISTOCHEMISTRY AND CELL BIOLOGY, SPRINGER, BERLIN, DE, vol. 147, no. 5, 23 December 2016 (2016-12-23), DE, pages 565 - 574, XP036217090, ISSN: 0948-6143, DOI: 10.1007/s00418-016-1531-7
ZHENG YAYUN, FENG YING, SU DONGMEI, ZHOU XUE, MA FANG: "Siglecs and Their Ligands Involved in Establishing Immune Tolerance at the Maternal-Fetal Interface", CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY, vol. 39, no. 4, 30 April 2019 (2019-04-30), CN, pages 316 - 320, XP009530068, ISSN: 0254-5101, DOI: 10.3760/cma.j.issn.0254-5101.2019.04.012
WEI DEHUA, WU QINGHUA, SHI HUIRONG: "Apoptosis and p53 expression in the placental villi of females with unexplained recurrent spontaneous abortion", EXPERIMENTAL AND THERAPEUTIC MEDICINE, SPANDIDOS PUBLICATIONS, GR, vol. 7, no. 1, 1 January 2014 (2014-01-01), GR, pages 191 - 194, XP055842132, ISSN: 1792-0981, DOI: 10.3892/etm.2013.1399
ZHENG YAYUN, FENG YING, SU DONGMEI, ZHOU XUE, MA FANG: "Siglecs and Their Ligands Involved in Establishing Immune Tolerance at the Maternal-Fetal Interface", CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY, vol. 39, no. 4, 30 April 2019 (2019-04-30), CN, pages 316 - 320, XP009530068, ISSN: 0254-5101, DOI: 10.3760/cma.j.issn.0254-5101.2019.04.012
WEI DEHUA, WU QINGHUA, SHI HUIRONG: "Apoptosis and p53 expression in the placental villi of females with unexplained recurrent spontaneous abortion", EXPERIMENTAL AND THERAPEUTIC MEDICINE, SPANDIDOS PUBLICATIONS, GR, vol. 7, no. 1, 1 January 2014 (2014-01-01), GR, pages 191 - 194, XP055842132, ISSN: 1792-0981, DOI: 10.3892/etm.2013.1399
Attorney, Agent or Firm:
TIANJIA PATENT OFFICE CHENGDU (GENERAL PARTNERSHIP) (CN)
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