Title:
METHOD FOR DETECTING STAPHYLOCOCCI IN MILK
Document Type and Number:
WIPO Patent Application WO/2015/093544
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a bacteriolysis method for detecting whether or not Staphylococci are causing mastitis using the milk of livestock, and a detection method and detection kit comprising a lysis solution and an immunochromatographic device. Provided is a bacteriolysis method for Staphylococci that includes a step for lysing Staphylococci present in milk by mixing, in milk obtained from livestock, a lytic agent containing lytic enzymes and at least one amphoteric surfactant, and, preferably, at least one non-ionic surfactant. The lytic enzymes are preferably lysostaphin.
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Inventors:
MAEHANA KOJI (JP)
MATSUYAMA KENJI (JP)
MATSUYAMA KENJI (JP)
Application Number:
PCT/JP2014/083459
Publication Date:
June 25, 2015
Filing Date:
December 17, 2014
Export Citation:
Assignee:
ASAHI CHEMICAL IND (JP)
International Classes:
C12N1/06; G01N33/48; G01N33/543; G01N33/569; C12Q1/04
Domestic Patent References:
| WO2005064016A1 | 2005-07-14 | |||
| WO2000006603A1 | 2000-02-10 |
Foreign References:
| JP2012122921A | 2012-06-28 | |||
| JPH0283336A | 1990-03-23 | |||
| JPH1128099A | 1999-02-02 | |||
| JP2003171291A | 2003-06-17 | |||
| JP2007146030A | 2007-06-14 | |||
| JP2010154851A | 2010-07-15 | |||
| JPH01244370A | 1989-09-28 | |||
| JP2012122921A | 2012-06-28 | |||
| JPH1128099A | 1999-02-02 | |||
| JP2006246792A | 2006-09-21 | |||
| JPS63167799A | 1988-07-11 |
Other References:
JOURNAL OF DAIRY RESEARCH, vol. 64, 1997, pages 253 - 260
See also references of EP 3085770A4
See also references of EP 3085770A4
Attorney, Agent or Firm:
SIKs & Co. (JP)
Patent business corporation patent firm Sykes (JP)
Patent business corporation patent firm Sykes (JP)
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