Title:
METHOD FOR DETECTING TARGET NUCLEIC ACID
Document Type and Number:
WIPO Patent Application WO/2021/199767
Kind Code:
A1
Abstract:
The present invention relates to a method for detecting a target nucleic acid, comprising: cutting a first flap of a first cleavage structure that is formed by a target nucleic acid, a first nucleic acid, and a second nucleic acid; cutting a second flap of a second cleavage structure that is formed by a third nucleic acid, the cut first flap, and a fourth nucleic acid; and detecting the presence of the target nucleic acid by detecting the cut second flap, wherein cutting the first flap and cutting the second flap are performed by cutting the first flap and the second flap with a flap endonuclease, and the flap endonuclease has an amino acid sequence having a sequence identity of 65% or higher with respect to the amino acid sequence of a flap endonuclease of a microbe selected from the group consisting of microbes belonging to Thermococcales and microbes belonging to Methanobacteriales.
Inventors:
MAKINO YOICHI (JP)
ISHINO YOSHIZUMI (JP)
ISHINO SONOKO (JP)
ISHINO YOSHIZUMI (JP)
ISHINO SONOKO (JP)
Application Number:
PCT/JP2021/006190
Publication Date:
October 07, 2021
Filing Date:
February 18, 2021
Export Citation:
Assignee:
TOPPAN INC (JP)
UNIV KYUSHU NAT UNIV CORP (JP)
UNIV KYUSHU NAT UNIV CORP (JP)
International Classes:
C12N15/31; C12N15/55; C12N15/63; C12Q1/34; C12Q1/68; C12Q1/6813
Domestic Patent References:
| WO2006011253A1 | 2006-02-02 | |||
| WO2019026884A1 | 2019-02-07 |
Foreign References:
| JP2007236209A | 2007-09-20 | |||
| JP2001518805A | 2001-10-16 |
Other References:
JOURNAL OF PRINTING SCIENCE AND TECHNOLOGY, vol. 54, no. 6, 2017, pages 377 - 382
See also references of EP 4092121A4
See also references of EP 4092121A4
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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