Title:
METHOD FOR DETECTING TARGET SUBSTANCE
Document Type and Number:
WIPO Patent Application WO/2023/080137
Kind Code:
A1
Abstract:
This method is for detecting a target substance (110) in a sample, and includes: a step for introducing into a well a sample, a first specifically binding substance (120) that is labeled with a first single-stranded nucleic acid fragment (121) and that is for the target substance (110), and a second specifically binding substance (130) that is labeled with a second single-stranded nucleic acid fragment (131) and that is for the target substance (110), so that if the target substance (110) is present in the sample, a complex (100) including the target substance (110), the first specifically binding substance (120), and the second specifically binding substance (130) is formed, and at least a portion of the first single-stranded nucleic acid fragment (121) and at least a portion of the second single-stranded nucleic acid fragment (131) hybridize to form double-stranded nucleic acid (140); and a step for detecting the formation of the double-stranded nucleic acid (140). The detection of the formation of the double-stranded nucleic acid (140) indicates the presence of the target substance (110).
Inventors:
KUBO YUJI (JP)
MAKINO YOICHI (JP)
MAKINO YOICHI (JP)
Application Number:
PCT/JP2022/040892
Publication Date:
May 11, 2023
Filing Date:
November 01, 2022
Export Citation:
Assignee:
TOPPAN INC (JP)
International Classes:
G01N33/536; C12N15/11; C12Q1/6813
Domestic Patent References:
| WO2010059820A1 | 2010-05-27 |
Foreign References:
| US20040248103A1 | 2004-12-09 | |||
| JP6183471B2 | 2017-08-23 | |||
| JP2008545142A | 2008-12-11 | |||
| JP2021519431A | 2021-08-10 | |||
| JP2020126068A | 2020-08-20 | |||
| JP2006511807A | 2006-04-06 | |||
| JP2021180020A | 2021-11-18 |
Other References:
MAKINO YOICHI, HIROSHI USHIDA: "Development of digital biomolecule detection system", JOURNAL OF PRINTING SCIENCE AND TECHNOLOGY, JAPANESE SOCIETY OF PRINTING SCIENCE AND TECHNOLOGY, JP, 1 January 2017 (2017-01-01), JP , pages 377 - 382, XP055952714, ISSN: 0914-3319, DOI: 10.11413/nig.54.377
KAN C. W. ET AL.: "Isolation and detection of single molecules on paramagnetic beads using sequential fluid flows in microfabricated polymer array assemblies", LAB ON A CHIP, vol. 12, no. 5, 2012, pages 977 - 985, XP055073933, DOI: 10.1039/C2LC20744C
MOHAMMED H. ET AL.: "Approaches for Assessing and Discovering Protein Interactions in Cancer", MOL CANCER RES, vol. 11, no. 11, 2013, pages 1295 - 1302, XP093135026, DOI: 10.1158/1541-7786.MCR-13-0454
ASSARSSON E. ET AL.: "Homogenous 96-Plex PEA Immunoassay Exhibiting High Sensitivity, Specificity, and Excellent Scalability", PLOS ONE, vol. 9, no. 4, 2014, pages e95192, XP055811932, DOI: 10.1371/journal.pone.0095192
KAN C. W. ET AL.: "Isolation and detection of single molecules on paramagnetic beads using sequential fluid flows in microfabricated polymer array assemblies", LAB ON A CHIP, vol. 12, no. 5, 2012, pages 977 - 985, XP055073933, DOI: 10.1039/C2LC20744C
MOHAMMED H. ET AL.: "Approaches for Assessing and Discovering Protein Interactions in Cancer", MOL CANCER RES, vol. 11, no. 11, 2013, pages 1295 - 1302, XP093135026, DOI: 10.1158/1541-7786.MCR-13-0454
ASSARSSON E. ET AL.: "Homogenous 96-Plex PEA Immunoassay Exhibiting High Sensitivity, Specificity, and Excellent Scalability", PLOS ONE, vol. 9, no. 4, 2014, pages e95192, XP055811932, DOI: 10.1371/journal.pone.0095192
Attorney, Agent or Firm:
MATSUNUMA Yasushi et al. (JP)
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