Title:
METHOD FOR THE DETECTION OF DNA SEQUENCE VARIATIONS
Document Type and Number:
WIPO Patent Application WO2002072877
Kind Code:
A3
Abstract:
The object of the current invention is the DNA analysis method which makes it possible to screen out all kinds of DNA variations, it means pointmutations, deletions, insertions, etc. The idea of this method is the restriction of analyzed DNA, the adding of specific DNA molecules (adaptor "a") to the DNA fragments, the dephosphorylation, dnaturation and renaturation of those adaptor-added DNA fragments, the filling of those adaptors (adaptor "a"). The enzymatic or chemical treatment of heteroduplex is being created during hybridization. The adding of specific DNA molecules (adaptros "b") to the free end of DNA fragments, generated during the eenzymatic or chemical treatment of heteroduplexis, and the amplification only of these DNA fragments which have different adaptors in both ends.
Inventors:
PUURAND UELO (EE)
Application Number:
PCT/EE2002/000002
Publication Date:
October 09, 2003
Filing Date:
February 22, 2002
Export Citation:
Assignee:
UNIV TARTU (EE)
PUURAND UELO (EE)
PUURAND UELO (EE)
International Classes:
C12Q1/68; C12Q1/683; (IPC1-7): C12Q1/68
Domestic Patent References:
| WO2000024935A2 | 2000-05-04 | |||
| WO2000053802A1 | 2000-09-14 |
Foreign References:
| US5811239A | 1998-09-22 | |||
| US5876941A | 1999-03-02 |
Other References:
KORTENKAMP ANDREAS ET AL: "Genotypic selection of mutated DNA sequences using mismatch cleavage analysis, a possible basis for novel mutation assays.", MUTAGENESIS, vol. 12, no. 5, 1997, pages 335 - 338, XP009010901, ISSN: 0267-8357
COLLASIUS M ET AL: "ANALYSIS OF UNKNOWN DNA SEQUENCES BY POLYMERASE CHAIN REACTION (PCR) USING A SINGLE SPECIFIC PRIMER AND A STANDARDIZED ADAPTOR", JOURNAL OF VIROLOGICAL METHODS, AMSTERDAM, NL, vol. 32, 1 April 1991 (1991-04-01), pages 115 - 119, XP000770770, ISSN: 0166-0934
TROUTT A B ET AL: "LIGATION-ANCHORED PCR: A SIMPLE AMPLIFICATION TECHNIQUE WITH SINGLE-SIDED SPECIFICITY", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 89, 1 October 1992 (1992-10-01), pages 9823 - 9825, XP002037449, ISSN: 0027-8424
ROUX K H ET AL: "A STRATEGY FOR SINGLE SITE PCR AMPLIFICATION OF DOUBLE STRANDED DNA PRIMING DIGESTED CLONED OR GENOMIC DNA FROM AN ANCHOR-MODIFIED RESTRICTION SITE AND A SHORT INTERNAL SEQUENCE", BIOTECHNIQUES, vol. 8, no. 1, 1990, pages 48 - 50, 52, 54, 56-57, XP009010860, ISSN: 0736-6205
MCALLISTER L ET AL: "ENRICHMENT FOR LOCI IDENTICAL-BY-DESCENT BETWEEN PAIRS OF MOUSE OR HUMAN GENOMES BY GENOMIC MISMATCH SCANNING", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 47, no. 1, 1998, pages 7 - 11, XP000929905, ISSN: 0888-7543
NELSON S F: "GNOMIC MISMATCH SCANNING: CURRENT PROGRESS AND POTENTIAL APPLICATIONS", ELECTROPHORESIS, WEINHEIM, DE, vol. 16, no. 2, 1995, pages 279 - 285, XP001070015, ISSN: 0173-0835
FORD M E ET AL: "Analysis of the hereditary pancreatitis-associated cationic trypsinogen gene mutations in exons 2 and 3 by enzymatic mutation detection from a single 2.2-kb polymerase chain reaction product.", MOLECULAR DIAGNOSIS: A JOURNAL DEVOTED TO THE UNDERSTANDING OF HUMAN DISEASE THROUGH THE CLINICAL APPLICATION OF MOLECULAR BIOLOGY. UNITED STATES SEP 1999, vol. 4, no. 3, September 1999 (1999-09-01), pages 211 - 218, XP009010893, ISSN: 1084-8592
LOWELL JENNIFER L ET AL: "Heteroduplex resolution using T7 endonuclease I in microbial community analyses.", BIOTECHNIQUES, vol. 28, no. 4, April 2000 (2000-04-01), pages 676 - 681, XP001147970, ISSN: 0736-6205
YOUIL R ET AL: "DETECTION OF 81 OF 81 KNOWN MOUSE BETA-GLOBIN PROMOTER MUTATIONS WITH T4 ENDONUCLEASE VII THE EMC METHOD", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 32, no. 3, 15 March 1996 (1996-03-15), pages 431 - 435, XP000600342, ISSN: 0888-7543
YOUIL R ET AL: "SCREENING FOR MUTATIONS BY ENZYME MISMATCH CLEAVAGE WITH T4 ENDONUCLEASE VII", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 92, 1995, pages 87 - 91, XP002011671, ISSN: 0027-8424
TAYLOR GRAHAM R: "Enzymatic and chemical cleavage methods.", ELECTROPHORESIS, vol. 20, no. 6, June 1999 (1999-06-01), pages 1125 - 1130, XP002241182, ISSN: 0173-0835
COLLASIUS M ET AL: "ANALYSIS OF UNKNOWN DNA SEQUENCES BY POLYMERASE CHAIN REACTION (PCR) USING A SINGLE SPECIFIC PRIMER AND A STANDARDIZED ADAPTOR", JOURNAL OF VIROLOGICAL METHODS, AMSTERDAM, NL, vol. 32, 1 April 1991 (1991-04-01), pages 115 - 119, XP000770770, ISSN: 0166-0934
TROUTT A B ET AL: "LIGATION-ANCHORED PCR: A SIMPLE AMPLIFICATION TECHNIQUE WITH SINGLE-SIDED SPECIFICITY", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 89, 1 October 1992 (1992-10-01), pages 9823 - 9825, XP002037449, ISSN: 0027-8424
ROUX K H ET AL: "A STRATEGY FOR SINGLE SITE PCR AMPLIFICATION OF DOUBLE STRANDED DNA PRIMING DIGESTED CLONED OR GENOMIC DNA FROM AN ANCHOR-MODIFIED RESTRICTION SITE AND A SHORT INTERNAL SEQUENCE", BIOTECHNIQUES, vol. 8, no. 1, 1990, pages 48 - 50, 52, 54, 56-57, XP009010860, ISSN: 0736-6205
MCALLISTER L ET AL: "ENRICHMENT FOR LOCI IDENTICAL-BY-DESCENT BETWEEN PAIRS OF MOUSE OR HUMAN GENOMES BY GENOMIC MISMATCH SCANNING", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 47, no. 1, 1998, pages 7 - 11, XP000929905, ISSN: 0888-7543
NELSON S F: "GNOMIC MISMATCH SCANNING: CURRENT PROGRESS AND POTENTIAL APPLICATIONS", ELECTROPHORESIS, WEINHEIM, DE, vol. 16, no. 2, 1995, pages 279 - 285, XP001070015, ISSN: 0173-0835
FORD M E ET AL: "Analysis of the hereditary pancreatitis-associated cationic trypsinogen gene mutations in exons 2 and 3 by enzymatic mutation detection from a single 2.2-kb polymerase chain reaction product.", MOLECULAR DIAGNOSIS: A JOURNAL DEVOTED TO THE UNDERSTANDING OF HUMAN DISEASE THROUGH THE CLINICAL APPLICATION OF MOLECULAR BIOLOGY. UNITED STATES SEP 1999, vol. 4, no. 3, September 1999 (1999-09-01), pages 211 - 218, XP009010893, ISSN: 1084-8592
LOWELL JENNIFER L ET AL: "Heteroduplex resolution using T7 endonuclease I in microbial community analyses.", BIOTECHNIQUES, vol. 28, no. 4, April 2000 (2000-04-01), pages 676 - 681, XP001147970, ISSN: 0736-6205
YOUIL R ET AL: "DETECTION OF 81 OF 81 KNOWN MOUSE BETA-GLOBIN PROMOTER MUTATIONS WITH T4 ENDONUCLEASE VII THE EMC METHOD", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 32, no. 3, 15 March 1996 (1996-03-15), pages 431 - 435, XP000600342, ISSN: 0888-7543
YOUIL R ET AL: "SCREENING FOR MUTATIONS BY ENZYME MISMATCH CLEAVAGE WITH T4 ENDONUCLEASE VII", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 92, 1995, pages 87 - 91, XP002011671, ISSN: 0027-8424
TAYLOR GRAHAM R: "Enzymatic and chemical cleavage methods.", ELECTROPHORESIS, vol. 20, no. 6, June 1999 (1999-06-01), pages 1125 - 1130, XP002241182, ISSN: 0173-0835
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