Title:
METHOD OF DETERMINING ABILITY OF DRUG TO BIND TO TROPONIN I AND TEST DRUG FOR THE DETERMINATION
Document Type and Number:
WIPO Patent Application WO/2005/106459
Kind Code:
A1
Abstract:
A method of screening a therapeutic drug for cardiac failure attributed to dilation failure, intended to facilitate the discovery of a drug for improving myocardial relaxation as a specific medicine, namely, a therapeutic drug for cardiac failure attributed to dilation failure. Also, there is provided a screening reagent therefor. In particular, there is provided a method of assaying the activity of drug on binding between actin/tropomyosin complex and troponin I, characterized in that the method comprises adding a test drug to an aqueous solution containing troponin I, troponin C, actin, tropomyosin and ATP; effecting reaction of the mixture; fractionating any precipitate from the reaction mixture; conducting separation of any precipitate lying in a liquid having any supernatant removed therefrom by polyacrylamide electrophoresis; staining separation bands with a colorant; and measuring the amount of separated matter in the separation band of troponin I.
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Inventors:
KANEKO NOBORU (JP)
Application Number:
PCT/JP2005/008565
Publication Date:
November 10, 2005
Filing Date:
April 28, 2005
Export Citation:
Assignee:
AETAS PHARMA CO LTD (JP)
KANEKO NOBORU (JP)
KANEKO NOBORU (JP)
International Classes:
A61K31/554; A61P9/00; A61P9/04; A61P9/10; A61P9/12; A61P11/00; A61P21/02; A61P43/00; C07D417/06; G01N33/15; G01N33/50; G01N33/68; C07D417/14; (IPC1-7): G01N33/15; A61K31/554; A61P9/00; A61P9/04; A61P9/10; A61P9/12; A61P11/00; A61P21/02; A61P43/00; C07D417/06; G01N33/50
Other References:
OHTSUKI I. ET AL: "Periodic Binding of Troponin C-I and Troponin I to Tropomyosin-Actin Filaments.", J.BIOCHEM., vol. 131, 2002, pages 739 - 743, XP002988436
SAI-MING NGAI ET AL: "Biologically Important Interactions between Synthetic Peptides of the N-terminal Region of Troponin I and Troponin C.", THE JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 267, no. 22, 1992, pages 15715 - 15720, XP002988450
SAI-MING NGAI ET AL: "Biologically Important Interactions between Synthetic Peptides of the N-terminal Region of Troponin I and Troponin C.", THE JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 267, no. 22, 1992, pages 15715 - 15720, XP002988450
Attorney, Agent or Firm:
Takeda, Masahiko (13-1, Nagata-cho 2-chome, Chiyoda-ku Tokyo, JP)
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