Title:
METHOD FOR DETERMINING ANTIGEN NEUTRALIZATION EQUIVALENT BASED ON ANTIGEN DETECTION REAGENT
Document Type and Number:
WIPO Patent Application WO/2023/124108
Kind Code:
A1
Abstract:
A method for determining an antigen neutralization equivalent based on an antigen detection reagent, specifically comprising the following steps: S1, antigen dilution: performing gradient dilution on an antigen with known purity and concentration by using a matrix to obtain a series of gradient diluent with different concentrations; S2, antigen and antibody incubation reaction: respectively adding an equivalent antibody standard substance or an antibody-containing sample into each gradient diluent with different concentrations obtained in step S1, and reacting to generate a mixed solution; and S3, determination of an antigen neutralization equivalent: adding a specific antigen detection reagent corresponding to the antigen in step S1 into each mixed solution obtained in step S2 for detection, and recording data so as to determine the antigen neutralization equivalent. The antigen neutralization equivalent is determined by using the antigen detection reagent, and the antibody is assigned by means of the antigen neutralization equivalent and the titer of the antibody, such that the method can be used for preparing an enterprise standard substance or even a national standard substance.
Inventors:
XU XINGSHANG (CN)
CHEN JEFFERY (CN)
WANG PENG (CN)
CHEN JEFFERY (CN)
WANG PENG (CN)
Application Number:
PCT/CN2022/112642
Publication Date:
July 06, 2023
Filing Date:
August 16, 2022
Export Citation:
Assignee:
LANSION BIOTECHNOLOGY CO LTD (CN)
International Classes:
G01N33/53; G01N33/96
Foreign References:
| CN114264808A | 2022-04-01 | |||
| CN113125756A | 2021-07-16 | |||
| CN112198318A | 2021-01-08 | |||
| CN110646614A | 2020-01-03 | |||
| CN101874206A | 2010-10-27 | |||
| JP2010237126A | 2010-10-21 | |||
| JPH075174A | 1995-01-10 |
Attorney, Agent or Firm:
NANJING ZHENGLIAN INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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