Title:
METHOD FOR DETERMINING FALSE NEGATIVE AND FALSE POSITIVE REACTIONS THAT CAN APPEAR IN DETECTION OF PATHOGEN GENE OF TARGET DISEASE, USING REAL-TIME RT-PCR METHOD FOR VIRAL HEMORRHAGIC SEPSIS AS STANDARD
Document Type and Number:
WIPO Patent Application WO/2022/039336
Kind Code:
A1
Abstract:
According to a method for determining false negative and false positive reactions that can appear in the detection of a pathogen gene of a target disease, using a real-time RT-PCR method for viral hemorrhagic sepsis as a standard, presented in the present invention, a synthetic gene including both a nucleotide sequence according to a standardized Jonstrup VHS rRT-PCR method and a nucleotide sequence for target disease detection is cloned into a structurally stable plasmid DNA vector to extract synthetic recombinant plasmid DNA, and detection sensitivities of real-time RT-PCR performed using the DNA are compared so that the detection sensitivity of a pathogen gene of a target disease can be verified, and a highly sensitive gene detection method for a target disease can be established in a short time by adjusting same.
Inventors:
KIM HYOUNG JUN (KR)
KIM YOUNG CHUL (KR)
KIM SHIN HU (KR)
KIM MIN JI (KR)
KIM YOUNG CHUL (KR)
KIM SHIN HU (KR)
KIM MIN JI (KR)
Application Number:
PCT/KR2021/000001
Publication Date:
February 24, 2022
Filing Date:
January 01, 2021
Export Citation:
Assignee:
REPUBLIC OF KOREA MINISTRY OF OCEANS AND FISHERIES NAT INSTITUTE OF FISHERIES SCIENCE (KR)
International Classes:
C12Q1/70; C12Q1/6806; C12Q1/6876
Foreign References:
| KR101782489B1 | 2017-09-28 | |||
| KR100942388B1 | 2010-02-17 | |||
| KR20140048496A | 2014-04-24 |
Other References:
WARG JV, CLEMENT T, CORNWELL ER, CRUZ A, GETCHELL RG, GIRAY C, GOODWIN AE, GROOCOCK GH, FAISAL M, KIM R, MERRY GE, PHELPS NBD, REI: "Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. I. Initial comparison of four protocols", DISEASES OF AQUATIC ORGANISMS, vol. 111, no. 1, 21 August 2014 (2014-08-21), DE , pages 1 - 13, XP055901839, ISSN: 0177-5103, DOI: 10.3354/dao02753
WARG JV, CLEMENT T, CORNWELL ER, CRUZ A, GETCHELL RG, GIRAY C, GOODWIN AE, GROOCOCK GH, FAISAL M, KIM R, MERRY GE, PHELPS NBD, REI: "Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. I. Initial comparison of four protocols", DISEASES OF AQUATIC ORGANISMS, vol. 111, no. 1, 21 August 2014 (2014-08-21), DE , pages 1 - 13, XP055901839, ISSN: 0177-5103, DOI: 10.3354/dao02753
WARG JV, CLEMENT T, CORNWELL ER, CRUZ A, GETCHELL RG, GIRAY C, GOODWIN AE, GROOCOCK GH, FAISAL M, KIM R, MERRY GE, PHELPS NBD, REI: "Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. I. Initial comparison of four protocols", DISEASES OF AQUATIC ORGANISMS, vol. 111, no. 1, 21 August 2014 (2014-08-21), DE , pages 1 - 13, XP055901839, ISSN: 0177-5103, DOI: 10.3354/dao02753
Attorney, Agent or Firm:
KIM, Keon Woo (KR)
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