Title:
A METHOD FOR DEVELOPING A TISSUE PROTEOME LIBRARY
Document Type and Number:
WIPO Patent Application WO2007080504
Kind Code:
B1
Abstract:
The present invention relates to the description of an approach for developing tissue proteome library, which overexpresses all the transcripts (mRNAs) present in a given tissue. Transcripts of interest present in a tissue are normally cloned and overexpressed individually to enable purification of expressed protein and for conducting its structure-function studies. Methods for identification of novel and low abundant transcripts present in tissues are not available, particularly of specimen tissue samples, oocytes and early embryos, for which tissue availability is also a serious limitation. Expression of all the transcripts present in a tissue and comparison of the profile of total expressed protein with that of appropriate controls can be used in identification of all and particularly novel transcripts present in a tissue. This novel proteome library construction approach enables expression of all the transcripts present in a tissue just in one go and analysis of all the expressed proteins employing proteomics and / or other suitable approaches.
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Inventors:
MURTHY SATYANARAYANA BULUSU (IN)
Application Number:
PCT/IB2007/000078
Publication Date:
December 27, 2007
Filing Date:
January 11, 2007
Export Citation:
Assignee:
COUNCIL SCIENT IND RES (IN)
MURTHY SATYANARAYANA BULUSU (IN)
MURTHY SATYANARAYANA BULUSU (IN)
International Classes:
C12N15/10; C12N15/62; C40B40/08; C40B40/10
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