Title:
METHOD FOR DISPLAYING LOOPS FROM IMMUNOGLOBULIN DOMAINS IN DIFFERENT CONTEXTS
Document Type and Number:
WIPO Patent Application WO2003050531
Kind Code:
A3
Abstract:
The present invention is related to an isolated polypeptide micro-scaffold displaying immunoglobulin CDR2 or CDR3 polypeptide sequences, comprising a CDR2 or CDR3 polypeptide sequence interconnecting fragments of the adjacent framework polypeptide sequences, which are arranged to form two anti-parallel beta -strands. The present invention is further related to a method to search, select or screen for immunoglobulin CDR2 or CDR3 polypeptide sequences that bind to a given antigen or mixture of antigens, comprising the steps of: Creating a CDR library with the method of claim 13 from the genetic information of an individual or group of individuals; Select a CDR, which binds to said antigen or mixture of antigens.
Inventors:
LASTERS IGNACE (BE)
PLETINCKX JURGEN (BE)
BOUTONNET NATHALIE (BE)
LAUWEREYS MARC (BE)
BEIRNAERT ELS (BE)
PLETINCKX JURGEN (BE)
BOUTONNET NATHALIE (BE)
LAUWEREYS MARC (BE)
BEIRNAERT ELS (BE)
Application Number:
PCT/BE2002/000189
Publication Date:
March 18, 2004
Filing Date:
December 11, 2002
Export Citation:
Assignee:
ALGONOMICS N V (BE)
ABLYNX NV (BE)
LASTERS IGNACE (BE)
PLETINCKX JURGEN (BE)
BOUTONNET NATHALIE (BE)
LAUWEREYS MARC (BE)
BEIRNAERT ELS (BE)
ABLYNX NV (BE)
LASTERS IGNACE (BE)
PLETINCKX JURGEN (BE)
BOUTONNET NATHALIE (BE)
LAUWEREYS MARC (BE)
BEIRNAERT ELS (BE)
International Classes:
C07K16/00; C07K16/28; C12N15/10; C12Q1/68; C40B30/04; C40B40/02; G01N33/68; (IPC1-7): C12Q1/68; G01N33/68; A61K39/395
Domestic Patent References:
| WO1998032845A1 | 1998-07-30 | |||
| WO2000034784A1 | 2000-06-15 | |||
| WO2001049713A2 | 2001-07-12 |
Other References:
BILLETTA, R. ET ZANETTI, M.: "Antigenicity and immunogenicity of antigenized antibodies. Studies on B and T cells.", INTERNATIONAL REVIEWS OF IMMUNOLOGY, vol. 10, no. 2-3, 1993, Switzerland, pages 251 - 263, XP009010620
ZHAO, B ET AL: "A paradigm for drug discovery using a conformation from the crystal structure of a presentation scaffold", NATURE STRUCTURAL BIOLOGY, vol. 2, no. 12, December 1995 (1995-12-01), USA, pages 1131 - 1137, XP009010617
KOIDE A ET AL: "THE FIBRONECTIN TYPE III DOMAIN AS A SCAFFOLD FOR NOVEL BINDING PROTEINS", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 284, 1998, pages 1141 - 1151, XP002944100, ISSN: 0022-2836
SODERLIND ESKIL ET AL: "Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries.", NATURE BIOTECHNOLOGY, vol. 18, no. 8, August 2000 (2000-08-01), pages 852 - 856, XP009010618, ISSN: 1087-0156
DESIDERIO, A. ET AL: "A semi-synthetic repertoire of intrinsically stable antibody fragments derived from a single-framework scaffold", J. MOL. BIOL., no. 310, 2001, pages 603 - 615, XP001152504
NUTTALL, S.D. ET AL: "Isolation of the antigen receptor from wobbegong sharks, and use as a scaffold for the display of protein loop libraries", MOLECULAR IMMUNOLOGY, no. 38, 2001, pages 313 - 326, XP001152503
ZHAO, B ET AL: "A paradigm for drug discovery using a conformation from the crystal structure of a presentation scaffold", NATURE STRUCTURAL BIOLOGY, vol. 2, no. 12, December 1995 (1995-12-01), USA, pages 1131 - 1137, XP009010617
KOIDE A ET AL: "THE FIBRONECTIN TYPE III DOMAIN AS A SCAFFOLD FOR NOVEL BINDING PROTEINS", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 284, 1998, pages 1141 - 1151, XP002944100, ISSN: 0022-2836
SODERLIND ESKIL ET AL: "Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries.", NATURE BIOTECHNOLOGY, vol. 18, no. 8, August 2000 (2000-08-01), pages 852 - 856, XP009010618, ISSN: 1087-0156
DESIDERIO, A. ET AL: "A semi-synthetic repertoire of intrinsically stable antibody fragments derived from a single-framework scaffold", J. MOL. BIOL., no. 310, 2001, pages 603 - 615, XP001152504
NUTTALL, S.D. ET AL: "Isolation of the antigen receptor from wobbegong sharks, and use as a scaffold for the display of protein loop libraries", MOLECULAR IMMUNOLOGY, no. 38, 2001, pages 313 - 326, XP001152503
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