Title:
METHOD FOR EDITING TARGET POLYNUCLEOTIDE AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/024119
Kind Code:
A1
Abstract:
A method for editing a target polynucleotide, comprising the specific steps of: designing a pair of DNA oligonucleotide single strands for a target gene of interest, such that the pair of single strands are respectively complementary to the sense and antisense strands of the target gene; respectively generating a structure recognizable by a type of nuclease which recognizes a target sequence structure and which has a cleaving activity, and cleaving a target genomic DNA by means of the nuclease, so as to edit a genome. The structure recognized by the type of nuclease which recognizes a target sequence structure and which has a cleaving activity is a 3'-Flap structure, the 3' ends of the DNA oligonucleotide single strands are not complementary to the target gene; a recombinant structure recognizes nucleic acid endonuclease and includes a structural recognition domain, a DNA cleavage domain, a peptide linking said two domains, and a nuclear localization signal.
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Inventors:
ZHOU GUOHUA (CN)
ZHAO QINGSHUN (CN)
XU SHU (CN)
CAO SHASHA (CN)
ZOU BINGJIE (CN)
YUE YUNYUN (CN)
ZHAO QINGSHUN (CN)
XU SHU (CN)
CAO SHASHA (CN)
ZOU BINGJIE (CN)
YUE YUNYUN (CN)
Application Number:
PCT/CN2017/093903
Publication Date:
February 08, 2018
Filing Date:
July 21, 2017
Export Citation:
Assignee:
UNIV NANJING (CN)
International Classes:
C12N15/113; C12N15/63
Foreign References:
| CN104745570A | 2015-07-01 | |||
| CN104531633A | 2015-04-22 |
Other References:
XU, S. ET AL.: "An alternative novel tool for DNA editing without target sequence limitation: the structure-guided nuclease", GENOME BIOLOGY, vol. 17, no. 186, 16 September 2016 (2016-09-16), pages 1 - 12, XP055459333
LI, L. ET AL.: "Functional domains in Fok I restriction endonuclease", PROC. NATL. ACAD. SCI. USA, vol. 89, 31 May 1992 (1992-05-31), pages 4275 - 4279, XP055459338
CHAPADOS, B.R. ET AL.: "Structural Basis for FEN-1 Substrate Specificity and PC-NA-Mediated Activation in DNA Replication and Repair", CELL, vol. 116, 9 January 2004 (2004-01-09), pages 39 - 50, XP055459343
KIM, H. ET AL.: "A guide to genome engineering with programmable nucleases", NATURE REVIEWS, vol. 15, 2 April 2014 (2014-04-02), pages 321 - 334, XP055177064
HSU, P.D. ET AL.: "Development and Applications of CRISPR-Cas9 for Genome Engineering", CELL, vol. 157, 5 June 2014 (2014-06-05), pages 1262 - 1277, XP028849523
HARRINGTON, J.J. ET AL.: "The characterization of a mammalian DNA structure-specific endonuclease", THE EMBO JOURNAL, vol. 13, no. 5, 31 December 1994 (1994-12-31), pages 1235 - 1246, XP002121673
LI, L. ET AL.: "Functional domains in Fok I restriction endonuclease", PROC. NATL. ACAD. SCI. USA, vol. 89, 31 May 1992 (1992-05-31), pages 4275 - 4279, XP055459338
CHAPADOS, B.R. ET AL.: "Structural Basis for FEN-1 Substrate Specificity and PC-NA-Mediated Activation in DNA Replication and Repair", CELL, vol. 116, 9 January 2004 (2004-01-09), pages 39 - 50, XP055459343
KIM, H. ET AL.: "A guide to genome engineering with programmable nucleases", NATURE REVIEWS, vol. 15, 2 April 2014 (2014-04-02), pages 321 - 334, XP055177064
HSU, P.D. ET AL.: "Development and Applications of CRISPR-Cas9 for Genome Engineering", CELL, vol. 157, 5 June 2014 (2014-06-05), pages 1262 - 1277, XP028849523
HARRINGTON, J.J. ET AL.: "The characterization of a mammalian DNA structure-specific endonuclease", THE EMBO JOURNAL, vol. 13, no. 5, 31 December 1994 (1994-12-31), pages 1235 - 1246, XP002121673
Attorney, Agent or Firm:
F&S INTELLECTUAL PROPERTY LTD. (CN)
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