Title:
METHOD FOR EFFICIENTLY ENRICHING LUTEIN FROM BROCCOLI SEEDLINGS USING γ-AMINOBUTYRIC ACID AND SODIUM CHLORIDE STRESS IN COMBINATION
Document Type and Number:
WIPO Patent Application WO/2021/232674
Kind Code:
A1
Abstract:
A method for efficiently enriching lutein from broccoli seedlings using γ-aminobutyric acid and sodium chloride stress in combination, comprising: taking Qingfeng variety of broccoli seeds having full grains, uniform sizes, and germinating capability as raw materials, removing impurities, performing disinfection and sterilization using 0.5% of NaClO, then shifting the raw materials to an incubator of 25℃ for germinating under 16 h light/8 h dark condition, spraying 0.25-1.00 mmol/L of γ-aminobutyric acid and 100 mM of NaCl water solution in combination after distilled water is sprayed for one day, and harvesting the broccoli seedlings four days later. By means of the method, the gene expression amounts of ZEP and VDE are respectively increased by 0.87-2.41 times and 1.14-2.75 times, and moreover, the content of the lutein in the broccoli seedlings is remarkably increased, thereby effectively enhancing the function of the broccoli seedlings and achieving broad application prospect.
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Inventors:
LI DAJING (CN)
HE WEIWEI (CN)
ZHOU ZHIYI (CN)
XIAO YADONG (CN)
SONG JIANGFENG (CN)
ZHANG ZHONGYUAN (CN)
HE WEIWEI (CN)
ZHOU ZHIYI (CN)
XIAO YADONG (CN)
SONG JIANGFENG (CN)
ZHANG ZHONGYUAN (CN)
Application Number:
PCT/CN2020/123608
Publication Date:
November 25, 2021
Filing Date:
October 26, 2020
Export Citation:
Assignee:
JIANGSU ACAD AGRICULTURAL SCI (CN)
International Classes:
A01G7/06
Foreign References:
| CN106922509A | 2017-07-07 | |||
| US20050181047A1 | 2005-08-18 |
Other References:
LUO HUANGYING, GAO HONG-BO,XIA QING-PING: "Effects of Exogenous GABA on Reactive Oxygen Species Metabolism and Chlorophyll Fluorescence Parameters in Tomato Seedlings Under NaCl Stress", HEBEI-NONGYE-DAXUE-XUEBAO = JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI, HEBEI-NONGYE-DAXUE, CN, vol. 34, no. 3, 15 May 2011 (2011-05-15), CN , XP055873480, ISSN: 1000-1573
HE LILONG, SHAN ZHONG-YING, GAO NA-NA, GUO SHANG-JING: "Effect of GABA on Germination of Wheat in Different Concentration of Salt", TIANJIN AGRICULTURAL SCIENCES, vol. 17, no. 3, 25 June 2011 (2011-06-25), pages 12 - 14, XP055873486, ISSN: 1006-6500, DOI: 10.3969/j.issn.1006-6500.2011.03.004
HE LILONG, SHAN ZHONG-YING, GAO NA-NA, GUO SHANG-JING: "Effect of GABA on Germination of Wheat in Different Concentration of Salt", TIANJIN AGRICULTURAL SCIENCES, vol. 17, no. 3, 25 June 2011 (2011-06-25), pages 12 - 14, XP055873486, ISSN: 1006-6500, DOI: 10.3969/j.issn.1006-6500.2011.03.004
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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