Title:
METHOD FOR EFFICIENTLY SCREENING FIBRINOGEN BY USING PHAGE ANTIBODY LIBRARY
Document Type and Number:
WIPO Patent Application WO/2020/259673
Kind Code:
A1
Abstract:
Disclosed is a method for efficiently screening fibrinogen by using a phage antibody library, comprising the steps of: coating a protein, sealing a flat plate, removing a background from a phage library, combining phage and the like. Compared with other phage, the phage screened by the present invention may be specifically combined with human fibrinogen, has a strong binding force and is easy to process. The phage may be added into various coagulation materials, the enrichment of fibrinogen is promoted by means of targeted combination of the human fibrinogen, and the blood coagulation effect is enhanced. The method has the characteristics of simple and easy to use reagents, a high success rate and the like.
Inventors:
YANG MINGYING (CN)
LEI FANG (CN)
SHUAI YAJUN (CN)
LEI FANG (CN)
SHUAI YAJUN (CN)
Application Number:
PCT/CN2020/098424
Publication Date:
December 30, 2020
Filing Date:
June 28, 2020
Export Citation:
Assignee:
UNIV ZHEJIANG (CN)
International Classes:
C40B30/04; A61P7/04; C07K7/08
Domestic Patent References:
| WO2003083436A2 | 2003-10-09 |
Foreign References:
| CN110453292A | 2019-11-15 |
Other References:
WU GUOQIU , WANG MING-HONG , ZHANG CHEN , LIU NAI-FENG: "Screening of phages specifically combined with connective tissue growth factor from a random 12-peptide library", JOURNAL OF CHINA PHARMACEUTICAL UNIVERSITY, vol. 38, no. 1, 25 February 2007 (2007-02-25), pages 87 - 91, XP055770494
XIANMIN BI: "Screening and function analysis of targeted short binding peptides of Endoglin", JOURNAL OF CHONGQING MEDICAL UNIVERSITY, vol. 32, no. 3, 30 March 2007 (2007-03-30), pages 232 - 235, XP055770502
XIANMIN BI: "Screening and function analysis of targeted short binding peptides of Endoglin", JOURNAL OF CHONGQING MEDICAL UNIVERSITY, vol. 32, no. 3, 30 March 2007 (2007-03-30), pages 232 - 235, XP055770502
Attorney, Agent or Firm:
HANGZHOU ZHONGCHENG PATENT AGENCY CO., LTD. (CN)
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