Title:
METHOD FOR ENHANCING FORMATION OF SPHEROID AND CULTURE MEDIUM FOR ENHANCING FORMATION OF SPHEROID
Document Type and Number:
WIPO Patent Application WO/2011/077894
Kind Code:
A1
Abstract:
Disclosed is a cell cultivation method which is capable of forming a spheroid by simple processes in a short term. Also disclosed is a culture medium for enhancing the formation of a spheroid, which can be used for the cell cultivation method. By culturing cells on a culture substrate, which has a recessed and projected structure that functions as a cell adhesion surface, using a cell culture medium that contains a predetermined extracellular matrix component, the proliferation and growth of monolayer cells as well as separation and collapse of a spheroid are prevented, thereby extremely effectively enhancing the formation of the spheroid.
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Inventors:
KOBAYASHI MAKI (JP)
YOSHIDA KAORI (JP)
WAKI ATSUO (JP)
TANAKA SATORU (JP)
YOSHII YUKIE (JP)
YOSHIDA KAORI (JP)
WAKI ATSUO (JP)
TANAKA SATORU (JP)
YOSHII YUKIE (JP)
Application Number:
PCT/JP2010/071002
Publication Date:
June 30, 2011
Filing Date:
November 25, 2010
Export Citation:
Assignee:
SCIVAX CORP (JP)
KOBAYASHI MAKI (JP)
YOSHIDA KAORI (JP)
WAKI ATSUO (JP)
TANAKA SATORU (JP)
YOSHII YUKIE (JP)
KOBAYASHI MAKI (JP)
YOSHIDA KAORI (JP)
WAKI ATSUO (JP)
TANAKA SATORU (JP)
YOSHII YUKIE (JP)
International Classes:
C12N5/00; C12N5/02
Foreign References:
JP2008022743A | 2008-02-07 | |||
JP4159103B2 | 2008-10-01 | |||
JP2009515558A | 2009-04-16 | |||
JP2008289362A | 2008-12-04 |
Other References:
MASAMI HIROYAMA ET AL.: "Nano Technology o Mochiita Kakushinteki 3-jigen Saibo Baiyoho (Nanoculture-ho) ni yoru Saisei Iryo eno Koken", EXPERIMENTAL MEDICINE, vol. 26, no. 18, 2008, pages 2942 - 2943
Attorney, Agent or Firm:
OKUDA Noritsugu (JP)
Okuda 律次 (JP)
Okuda 律次 (JP)
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