Title:
METHOD FOR ENRICHING PLANT MITOCHONDRIA BY USING GRADING MESH SIEVE
Document Type and Number:
WIPO Patent Application WO/2020/103182
Kind Code:
A1
Abstract:
Disclosed is a method for enriching plant mitochondria by using a grading mesh sieve. The method is primarily based on the characteristics of plant organelles and grading mesh sieves, and uses a combination of mesh sieves having different mesh sizes to selectively grade and filter plant organelles, thereby enriching and extracting plant mitochondria. This method has strong selectivity, targeting and specificity with respect to mitochondrial enrichment. During the enrichment process, the mitochondria are in an isotonic state and are not subjected to severe centrifugal operations and shaking. The extraction process is mild to effectively reduce loss of mitochondria and improves the recovery and wholeness of mitochondria during the extraction process.
Inventors:
DUAN NAIBIN (CN)
WANG XIAOMU (CN)
DING HANFENG (CN)
MA YUMIN (CN)
GONG YONGCHAO (CN)
XIE KUN (CN)
BAI JING (CN)
YANG YONGYI (CN)
WANG XIAOMU (CN)
DING HANFENG (CN)
MA YUMIN (CN)
GONG YONGCHAO (CN)
XIE KUN (CN)
BAI JING (CN)
YANG YONGYI (CN)
Application Number:
PCT/CN2018/118581
Publication Date:
May 28, 2020
Filing Date:
November 30, 2018
Export Citation:
Assignee:
CROP GERMPLASM RESOURCE CENTER OF SHANDONG PROVINCE (CN)
International Classes:
C12N5/04
Foreign References:
| CN106947729A | 2017-07-14 | |||
| CN107266521A | 2017-10-20 | |||
| CN108728396A | 2018-11-02 | |||
| CN105296324A | 2016-02-03 | |||
| CN108373988A | 2018-08-07 |
Attorney, Agent or Firm:
JINAN CHENGZHI TRADEMARK & PATENT OFFICE CO., LTD. (CN)
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