Title:
METHOD FOR ENZYMATIC PREPARATION OF FLUDARABINE PHOSPHATE
Document Type and Number:
WIPO Patent Application WO/2017/124315
Kind Code:
A1
Abstract:
A method for enzymatic preparation of fludarabine phosphate, comprising reaction of fludarabine with a high-energy phosphate compound under the action of deoxyribonucleic acid kinase. According to said method, acetate kinase and acetyl phosphate free acid or acetyl phosphate are also added. The technical problems present in the existing processes are successfully addressed by employing the enzymatic process to prepare the fludarabine phosphate. The usage of the high-energy phosphate compound is reduced by means of adding acetate kinase to recycle and regenerate a small amount of the high-energy phosphate compound, thereby reducing the generation of by-products having similar structures to the fludarabine phosphate, enhancing the operation convenience of purification steps in the industrial production of the fludarabine phosphate. The process is environment friendly, the reaction conditions are moderate, the cost is low, and the yield and the purity of the product obtained are high.
Inventors:
FU DEJIN (CN)
YANG YONG (CN)
YAN WEI (CN)
WEN WEIJIANG (CN)
GU XIANDE (CN)
CHEN HUA (CN)
MA XIAOJUN (CN)
ZHANG LING (CN)
WANG JUNKAI (CN)
YANG YONG (CN)
YAN WEI (CN)
WEN WEIJIANG (CN)
GU XIANDE (CN)
CHEN HUA (CN)
MA XIAOJUN (CN)
ZHANG LING (CN)
WANG JUNKAI (CN)
Application Number:
PCT/CN2016/071419
Publication Date:
July 27, 2017
Filing Date:
January 20, 2016
Export Citation:
Assignee:
ZHEJIANG HISUN PHARM CO LTD (CN)
International Classes:
C07H19/20; C12P19/30; C12P19/32
Domestic Patent References:
| WO2007144168A1 | 2007-12-21 |
Foreign References:
| US5700666A | 1997-12-23 |
Other References:
SERRA, I. ET AL.: "Immobilized Drosophila melanogaster Deoxyribonucleoside Kinase (DmdNK) as a High Performing Biocatalyst for the Synthesis of Purine Arabinonucleotides", ADVANCED SYNTHESIS & CATALYSIS, vol. 365, no. 2-3, 6 February 2014 (2014-02-06), pages 563 - 570, XP055400565
BARAI, V.N. ET AL.: "An Improved Method for the Enzymatic Transformation of Nucleosides into 5'-Monophosphates", BIOTECHNOLOGY LETTERS, vol. 26, no. 24, 31 December 2004 (2004-12-31), pages 1847 - 1850, XP019230815
See also references of EP 3406729A4
BARAI, V.N. ET AL.: "An Improved Method for the Enzymatic Transformation of Nucleosides into 5'-Monophosphates", BIOTECHNOLOGY LETTERS, vol. 26, no. 24, 31 December 2004 (2004-12-31), pages 1847 - 1850, XP019230815
See also references of EP 3406729A4
Attorney, Agent or Firm:
BOSS & YOUNG PATENT AND TRADEMARK LAW OFFICE (CN)
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