METHOD FOR ENZYMATICALLY ASSAYING SACCHARIFICATION PROTEIN

Title:

METHOD FOR ENZYMATICALLY ASSAYING SACCHARIFICATION PROTEIN

Document Type and Number:

WIPO Patent Application WO/1998/048043

Kind Code:

A1

Abstract:

FAOD (fructosylamino acid oxidase) is reacted with a sample containing a saccharification protein after or while treating the sample with protease XIV or a protease derived from the genus Aspergillus to determine the amount of oxygen consumed in the FAOD reaction or the amount of the reaction product, thereby assaying saccharification protein. According to the above method, the saccharification protein is fragmented by the above protease while inhibiting the decomposition of degradation of FAOD per se, thus facilitating bonding of the protein to FAOD, which improves the detection sensitivity.

Inventors:

ISHIMARU KAORI (JP)
OOISHI KATSUTAKA (JP)
FUKUYA HIROSHI (JP)
OKAMURA AKIHIKO (JP)

Application Number:

PCT/JP1998/001904

Publication Date:

October 29, 1998

Filing Date:

April 24, 1998

Export Citation:

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Assignee:

KYOTO DAIICHI KAGAKU KK (JP)
ISHIMARU KAORI (JP)
OISHI KATSUTAKA (JP)
FUKUYA HIROSHI (JP)
OKAMURA AKIHIKO (JP)

International Classes:

C12N9/62; C12Q1/26; C12Q1/37; (IPC1-7): C12Q1/26; C12N9/62

Foreign References:

JPS61280297A	1986-12-10
JPH05192193A	1993-08-03
JPH07289253A	1995-11-07
JPH08154672A	1996-06-18

Other References:

LUISETTI M., ET AL.: "SOME PROPERTIES OF THE ALKALINE PROTEINASE FROM ASPERGILLUS MELLEUS", INTERNATIONAL JOURNAL OF TISSUE REACTIONS., BIOSCIENCE EDIPRINT, GENEVA., CH, vol. 13., no. 04., 1 January 1991 (1991-01-01), CH, pages 187 - 192., XP002912905, ISSN: 0250-0868
See also references of EP 0921198A4

Attorney, Agent or Firm:

Hasegawa, Yoshiki (6F. Kyobashi National Building, 13-10, Kyobashi 2-chom, Chuo-ku Tokyo, JP)

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