Title:
METHOD FOR ESTIMATING MICROBIAL GROWTH RATE IN FOOD, METHOD FOR EVALUATING SHELF LIFE OF FOOD, AND SYSTEM FOR ESTIMATING MICROBIAL GROWTH RATE IN FOOD
Document Type and Number:
WIPO Patent Application WO/2023/190864
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for estimating the rate of microbial growth in a food that enables non-destructive and quick evaluation of the shelf life (for example, best before date), etc. of the food while reducing the cost and labor required for the evaluation. To solve this problem, provided is a method comprising: a step for placing a hydrophilic membrane filter 6 on the surface of a food sample 5 and inoculating a microorganism 7 on the hydrophilic membrane filter 6; a step for imaging the microorganism 7 growing on the hydrophilic membrane filter 6 over time using a microscope; a step for quantifying the area of the microorganism 7 from the images taken over time; and a step for estimating the growth rate of the microorganism 7 on the basis of the quantified data.
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Inventors:
TSUDA KENTARO (JP)
TAMIYA HISATO (JP)
TAMIYA HISATO (JP)
Application Number:
PCT/JP2023/013171
Publication Date:
October 05, 2023
Filing Date:
March 30, 2023
Export Citation:
Assignee:
NIPPON SHINYAKU CO LTD (JP)
International Classes:
C12M1/34; C12Q1/06; G01N33/02; C12N1/16
Foreign References:
| JP2018042502A | 2018-03-22 | |||
| JP2004344087A | 2004-12-09 | |||
| JP2010246442A | 2010-11-04 | |||
| JP2007195454A | 2007-08-09 |
Other References:
UBALDI M RODRIGUES, R .G. KROLL: "Rapid selective enumeration of bacteria in foods using a microcolony epifluoresccnce microscopy technique", JOURNAL O{ APPLIED BACTERIOLOGY, vol. 64, 1 January 1988 (1988-01-01), pages 65 - 78, XP055616169
Attorney, Agent or Firm:
SAITOH Yukihiko et al. (JP)
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