Title:
METHOD FOR EVALUATING ADAPTER LIGATION EFFICIENCY IN SEQUENCE OF DNA SAMPLE
Document Type and Number:
WIPO Patent Application WO/2022/131285
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for conveniently and accurately evaluating the ligation efficiency in a sequence of a DNA sample in order to optimize the condition of ligating Y-type adapters to both ends of a double-stranded DNA fragment. The present invention relates to a method for evaluating the efficiency of ligation reaction through which Y-type adapters are ligated to both ends of DNA to be analyzed, in a sequence of the DNA to be analyzed using the Y-type adapter, wherein the efficiency of reaction is evaluated by electrophoresing a reaction mixture containing ligation molecules, between the DNA and the Y-type adapters, produced by the ligation reaction under a predetermined condition, and analyzing a band separated on the basis of the number of adapters ligated to the DNA.
Inventors:
TANAKA MASAFUMI (JP)
INOKO HIDETOSHI (JP)
INOKO HIDETOSHI (JP)
Application Number:
PCT/JP2021/046211
Publication Date:
June 23, 2022
Filing Date:
December 15, 2021
Export Citation:
Assignee:
GENODIVE PHARMA INC (JP)
International Classes:
C12N15/09; C12Q1/6844; C12Q1/6869
Foreign References:
| JP2020516281A | 2020-06-11 | |||
| JP6685324B2 | 2020-04-22 | |||
| JP2019504624A | 2019-02-21 |
Attorney, Agent or Firm:
UCHIDA, Naoto (JP)
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