Title:
METHOD FOR EVALUATING CARDIOCYTES USING RAMAN SCATTERING
Document Type and Number:
WIPO Patent Application WO/2023/136308
Kind Code:
A1
Abstract:
Provided are methods for evaluating differentiation into cardiocytes and maturation of the cardiocytes. Method for evaluating cardiocytes using Raman scattering: The Raman spectrum of cardiocytes artificially induced to differentiate from pluripotent stem cells is acquired, the intensity of Raman-scattered light for proteins that include heme b and/or heme c as prosthetic groups is acquired according to the Raman spectrum, and the state of progress of maturation of the cardiocytes is evaluated on the basis of the intensity of the Raman-scattered light. Method for evaluating differentiation into cardiocytes using Raman scattering: Differentiation of pluripotent stem cells into cardiocytes is artificially induced, the Raman spectrum of the cell for which differentiation was induced is acquired, the intensity of Raman-scattered light for heme b and/or heme c is acquired according to the Raman spectrum, and the state of progress of differentiation into cardiocytes is evaluated on the basis of the intensity of the Raman-scattered light.
Inventors:
FUJITA KATSUMASA (JP)
BANDO KAZUKI (JP)
LIU LI (JP)
LI JUNJUN (JP)
SAWA YOSHIKI (JP)
MIYAGAWA SHIGERU (JP)
NAWA YASUNORI (JP)
FUJITA SATOSHI (JP)
BANDO KAZUKI (JP)
LIU LI (JP)
LI JUNJUN (JP)
SAWA YOSHIKI (JP)
MIYAGAWA SHIGERU (JP)
NAWA YASUNORI (JP)
FUJITA SATOSHI (JP)
Application Number:
PCT/JP2023/000671
Publication Date:
July 20, 2023
Filing Date:
January 12, 2023
Export Citation:
Assignee:
UNIV OSAKA (JP)
AIST (JP)
AIST (JP)
International Classes:
G01N21/65; C12Q1/06
Domestic Patent References:
| WO2020162601A1 | 2020-08-13 | |||
| WO2012059748A1 | 2012-05-10 |
Foreign References:
| JP2014224724A | 2014-12-04 | |||
| JP2014228321A | 2014-12-08 | |||
| JP2019504290A | 2019-02-14 | |||
| JP2021170250A | 2021-10-28 | |||
| JP2015062377A | 2015-04-09 |
Attorney, Agent or Firm:
IEIRI Takeshi (JP)
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