Title:
METHOD FOR EVALUATING A COATING STATE OF EITHER AN ADSORBENT OF A SAMPLE PROTEIN OR AN ADSORPTION STATE OF A SAMPLE PROTEIN
Document Type and Number:
WIPO Patent Application WO/2021/054262
Kind Code:
A1
Abstract:
A method for evaluating a coating state of either an adsorbent of a sample protein (40) or an adsorption state of the sample protein, the method comprising: providing a polymer substrate 1 (10) having a coating layer (20) containing the adsorbent of the sample protein and a fluorescent dye formed on at least one surface of the polymer substrate, and irradiating the substrate 1 with light to detect a color state 1 of the substrate 1; providing a polymer substrate 2 having a first coating layer (20) containing the adsorbent of the sample protein and a fluorescent dye and a second coating layer (30) containing a protein detection reagent (31) which conjugates with the sample protein, the first and second coating layers being sequentially formed on at least one surface of the polymer substrate, and irradiating the substrate 2 with light to detect a color state 2 of the substrate 2; providing a polymer substrate 3 having a coating layer containing the adsorbent of the sample protein and a fluorescent dye formed on at least one surface of the polymer substrate, introducing a sample containing the sample protein, which absorbs the irradiated light, onto the substrate 3 to obtain a substrate 4, and irradiating the substrate 4 with light to detect a color state 3 of the substrate 4; providing a polymer substrate 5 having a coating layer containing the adsorbent of the sample protein and a fluorescent dye formed on at least one surface of the polymer substrate, introducing a sample containing the sample protein (40) onto the substrate 5 to obtain a substrate 6, introducing the protein detection reagent (31) which has a higher degree of light absorption than that of the sample protein and which conjugates with the sample protein onto the polymer substrate 6 to obtain a substrate 7, and irradiating the substrate 7 with light to detect a color state 4 of the substrate 7;and comparing the color state 4 with the color states 1, 2 or 3 to evaluate a coating state of the adsorbent of the sample protein or the adsorption state of the sample protein.
Inventors:
XU ZHE (JP)
ANZAI TAKAO (JP)
ANZAI TAKAO (JP)
Application Number:
PCT/JP2020/034523
Publication Date:
March 25, 2021
Filing Date:
September 11, 2020
Export Citation:
Assignee:
TERUMO CORP (JP)
International Classes:
G01N21/77; G01N21/64; G01N21/78; G01N33/543
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Attorney, Agent or Firm:
HATTA & ASSOCIATES (JP)
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