Title:
METHOD FOR EVALUATING QUALITY OF HUMAN MESENCHYMAL STEM CELL, AND MONOCLONAL ANTIBODY FOR USE IN SAID METHOD
Document Type and Number:
WIPO Patent Application WO/2016/017795
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: a method for evaluating the quality of a human mesenchymal stem cell; a method for separating, selecting and culturing a human mesenchymal stem cell; a human mesenchymal stem cell population which can proliferate rapidly; and a monoclonal antibody which can specifically recognize a human mesenchymal stem cell that proliferates rapidly. Human mesenchymal stem cells that proliferate rapidly are separated and selected from a cell population containing human mesenchymal stem cells, and are then cultured. The abundance ratio of cells each having Ror2 or Fzd5 expressed therein in the cell population that has been separated, selected and cultured is quantified to determine whether or not the cell population passes or fails.
Inventors:
IYOKU YUMI (JP)
OKANO HIDEYUKI (JP)
MABUCHI YO (JP)
OKANO HIDEYUKI (JP)
MABUCHI YO (JP)
Application Number:
PCT/JP2015/071770
Publication Date:
February 04, 2016
Filing Date:
July 31, 2015
Export Citation:
Assignee:
IYOKU YUMI (JP)
International Classes:
C12N5/0775; C07K16/28; C12N15/02; C12P1/00; C12Q1/04; C12P21/08
Foreign References:
| JP2009527485A | 2009-07-30 | |||
| JP2009513708A | 2009-04-02 | |||
| JP2009060840A | 2009-03-26 | |||
| JP2006524508A | 2006-11-02 |
Other References:
MABUCHI Y. ET AL.: "LNGFR+THY-I+VCAM-Ihi+ cells reveal functionally distinct subpopulations in mesenchymal stem cells", STEM CELL REPORTS, vol. 1, 2013, pages 152 - 165, XP055391281
KEN'ICHI MIYAMOTO ET AL.: "Hito Kan'yokei Kansaibo Tokuiteki miRNA ni yoru Mibunka Jotai Iji Kiko no Kaimei", REGENERATIVE MEDICINE SPECIAL EXTRA ISSUE, vol. 13, 7 January 2014 (2014-01-07), pages 317, XP008185709
PITTENGER M ET AL.: "Multilineage potential of adult human mesenchymal stem cells", SCIENCE, vol. 284, 1999, pages 143 - 147, XP000867221, DOI: doi:10.1126/science.284.5411.143
SHEN Y ET AL.: "Bone marrow mesenchymal stem cell -derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non- canonical Wnt signaling pathway", ONCOLOGY LETTERS, vol. 8, pages 85 - 90, XP055391287
XIN H ET AL.: "The Wnt5a/Ror2 pathway is associated with determination of the differentiation fate of bone marrow mesenchymal stem cells invascular calcification", INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, vol. 31, 2013, pages 583 - 588, XP055391291
See also references of EP 3176253A4
KEN'ICHI MIYAMOTO ET AL.: "Hito Kan'yokei Kansaibo Tokuiteki miRNA ni yoru Mibunka Jotai Iji Kiko no Kaimei", REGENERATIVE MEDICINE SPECIAL EXTRA ISSUE, vol. 13, 7 January 2014 (2014-01-07), pages 317, XP008185709
PITTENGER M ET AL.: "Multilineage potential of adult human mesenchymal stem cells", SCIENCE, vol. 284, 1999, pages 143 - 147, XP000867221, DOI: doi:10.1126/science.284.5411.143
SHEN Y ET AL.: "Bone marrow mesenchymal stem cell -derived Wnt5a inhibits leukemia cell progression in vitro via activation of the non- canonical Wnt signaling pathway", ONCOLOGY LETTERS, vol. 8, pages 85 - 90, XP055391287
XIN H ET AL.: "The Wnt5a/Ror2 pathway is associated with determination of the differentiation fate of bone marrow mesenchymal stem cells invascular calcification", INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, vol. 31, 2013, pages 583 - 588, XP055391291
See also references of EP 3176253A4
Attorney, Agent or Firm:
KITAMURA, Koji et al. (JP)
Koji Kitamura (JP)
Koji Kitamura (JP)
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