Title:
METHOD FOR EVALUATING VIRUS INFECTION
Document Type and Number:
WIPO Patent Application WO/2023/063355
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method of highly-sensitive detection of adeno-associated virus (AAV)-neutralizing antibody, and providing a tool (e.g., DNA, cells) used in said method. Specifically, the present invention addresses the problem of providing a vector composition for AAV-neutralizing antibody detection, the composition containing vectors of (a), (b) and (c) below. (a) A vector comprising a DNA that includes a 5' transposon-specific inverted terminal repeat (ITR) sequence (hereinafter referred to as "5'ITR sequence"), a promoter, a transcription termination STOP sequence that can be deleted by a site-specific recombinant enzyme, one or more reporter genes, and a 3' transposon ITR sequence (hereinafter referred to as "3'ITR sequence"), which are connected in this order, the DNA being configured such that if the transcription termination STOP sequence is deleted, the one or more reporter genes are expressed by said promoter (hereinafter referred to as "reporter gene expressing cassette"); (b) a vector comprising a DNA that includes a 5'ITR sequence, a promoter, a selection marker gene, and a 3'ITR sequence, which are connected in this order, the DNA being configured such that said selection marker gene can be expressed by said promotor (hereinafter referred to as a "selection marker gene expressing cassette"); and (c) a vector comprising a DNA in which a promotor, and a transposase gene that recognizes the transposon ITR sequences included in vectors (a) and (b), are connected in this order (hereinafter referred to as "transposase gene-expressing cassette"), the DNA being configured such that said transposase gene can be expressed by said promoter.
Inventors:
OKADA TAKASHI (JP)
TSUNEKAWA YUJI (JP)
TSUNEKAWA YUJI (JP)
Application Number:
PCT/JP2022/038051
Publication Date:
April 20, 2023
Filing Date:
October 12, 2022
Export Citation:
Assignee:
UNIV TOKYO (JP)
International Classes:
C12N15/864; C12N5/10; C12Q1/02; C12Q1/66
Domestic Patent References:
| WO2017096162A1 | 2017-06-08 |
Foreign References:
| JP2018524287A | 2018-08-30 |
Other References:
NAKANISHI HIDEYUKI, HIGUCHI YURIKO, KAWAKAMI SHIGERU, YAMASHITA FUMIYOSHI, HASHIDA MITSURU: "Development and Therapeutic Application of Transposon-based Vectors", YAKUGAKU ZASSHI : JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, PHARMACEUTICAL SOCIETY OF JAPAN, vol. 129, no. 12, 1 December 2009 (2009-12-01), pages 1433 - 1443, XP093057029, ISSN: 0031-6903, DOI: 10.1248/yakushi.129.1433
ERIC KOWARZ; DENISE LÖSCHER; ROLF MARSCHALEK: "Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines", BIOTECHNOLOGY JOURNAL, WILEY-VCH VERLAG, WEINHEIM, DE, vol. 10, no. 4, 2 March 2015 (2015-03-02), DE , pages 647 - 653, XP072402683, ISSN: 1860-6768, DOI: 10.1002/biot.201400821
ERIC KOWARZ; DENISE LÖSCHER; ROLF MARSCHALEK: "Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines", BIOTECHNOLOGY JOURNAL, WILEY-VCH VERLAG, WEINHEIM, DE, vol. 10, no. 4, 2 March 2015 (2015-03-02), DE , pages 647 - 653, XP072402683, ISSN: 1860-6768, DOI: 10.1002/biot.201400821
Attorney, Agent or Firm:
OKUHARA Koji (JP)
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