Title:
METHOD FOR EXPLORING USEFUL GENETIC RESOURCES THROUGH BULK METAGENOME ANALYSIS AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/209037
Kind Code:
A1
Abstract:
The present invention relates to a degenerate primer for analyzing bulk metagenome information, and a method and a kit for analyzing metagenome information using the same. The method for analyzing metagenome information, according to the present invention, and a primer set used therefor can be applied to the present invention in order to rapidly determine the utility value of extensive metagenome samples. In particular, a superfamily-specific degenerate primer of the present invention can be used to rapidly determine the presence or absence of genetic information of target peptides in a metagenome by a simple method, and therefore can be used to collect large amounts of useful peptide resource information at a high speed from various metagenome samples. In addition, based on the method of the present invention, it can be used to explore a new peptide gene through design and manufacturing of a superfamily-specific degenerate primer of new target peptides. In addition, the method of the present invention can be applied to research relating to polypeptides, oligopeptides, antibiotic resistant genes, antimicrobial peptides, anti-fungal peptides, oligopeptides, markers, or Single-Nucleotide Polymorphism (SNP), as well as enzymes.
Inventors:
LEE DONG WOO (KR)
LEE YONG JIK (KR)
LEE SANG JAE (KR)
LEE HAN SEUNG (KR)
LEE YONG JIK (KR)
LEE SANG JAE (KR)
LEE HAN SEUNG (KR)
Application Number:
PCT/KR2016/006792
Publication Date:
December 29, 2016
Filing Date:
June 24, 2016
Export Citation:
Assignee:
KYUNGPOOK NAT UNIV INDUSTRY-ACADEMIC COOP FOUND (KR)
International Classes:
C12Q1/68
Foreign References:
| US7872100B2 | 2011-01-18 | |||
| KR20110073049A | 2011-06-29 | |||
| JP2014103948A | 2014-06-09 |
Other References:
ISERTE ET AL.: "Family-specific Degenerate Primer Design: a Tool to Design Consensus Degenerated Oligonucleotides", BIOTECHNOLOGY RESEARCH INTERNATIONAL, vol. 2013, 21 February 2013 (2013-02-21), pages 1 - 9, XP055341071
JARIC ET AL.: "Better Primer Design for Metagenomics Applications by Increasing Taxonomic Distinguishability", BMC PROCEEDINGS, vol. 7, no. supplement 7, 20 December 2013 (2013-12-20), pages 1 - 10, XP021171620
JARIC ET AL.: "Better Primer Design for Metagenomics Applications by Increasing Taxonomic Distinguishability", BMC PROCEEDINGS, vol. 7, no. supplement 7, 20 December 2013 (2013-12-20), pages 1 - 10, XP021171620
Attorney, Agent or Firm:
KIM, SOON WOONG (KR)
κΉμμ (KR)
κΉμμ (KR)
Download PDF: