Title:
METHOD FOR EXTRACTING AND ISOLATING LUTEIN CRYSTAL FROM VEGETABLE OIL RESIN CONTAINING LUTEIN DIESTER
Document Type and Number:
WIPO Patent Application WO/2020/011176
Kind Code:
A1
Abstract:
The present invention provides a method for extracting and isolating a lutein crystal from a vegetable oil resin containing a lutein diester, comprising the following steps: a) dissolving lipase into deionized water to form an enzyme solution; b) dissolving a lutein extract into an alcohol solvent containing the deionized water to form a uniform alcohol solution; c) adding the enzyme solution to the alcohol solution for performing hydrolysis, and stirring same to obtain a lutein solution; d) filtering and performing filtration isolation on the lutein solution to obtain a crystalline; e) re-dissolving the crystalline into a non-polar organic solvent, and using the deionized water for washing a water-soluble impurity; f) recycling the organic solvent and cooling same to obtain a recrystallization; and g) isolating the recrystallization and drying same to obtain the lutein crystal. According to the present invention, the selectivity is strong, the reaction time is short, no waste water is produced, the process is environment-friendly, the obtained lutein crystal is high in purity and yield, and the method is suitable for industrial production.
Inventors:
XU XINDE (CN)
XIE TIAN (CN)
WANG SHENGFAN (CN)
WANG QIUYAN (CN)
ZHENG JIANYONG (CN)
ZENG ZHAOWU (CN)
YIN XIAOPU (CN)
LAO XUEJUN (CN)
SHAO KANGZHONG (CN)
XIE TIAN (CN)
WANG SHENGFAN (CN)
WANG QIUYAN (CN)
ZHENG JIANYONG (CN)
ZENG ZHAOWU (CN)
YIN XIAOPU (CN)
LAO XUEJUN (CN)
SHAO KANGZHONG (CN)
Application Number:
PCT/CN2019/095327
Publication Date:
January 16, 2020
Filing Date:
July 09, 2019
Export Citation:
Assignee:
ZHEJIANG MED XINCHANG PHARM (CN)
International Classes:
C07C403/24
Foreign References:
| CN107550894A | 2018-01-09 | |||
| US3783099A | 1974-01-01 | |||
| CN102796715A | 2012-11-28 | |||
| US5382714A | 1995-01-17 | |||
| US5648564A | 1997-07-15 | |||
| US6262284B1 | 2001-07-17 | |||
| US6380442B1 | 2002-04-30 | |||
| US6743953B2 | 2004-06-01 | |||
| CN101532045A | 2009-09-16 | |||
| CN101235409A | 2008-08-06 | |||
| CN107475343A | 2017-12-15 | |||
| CN102796715A | 2012-11-28 |
Other References:
LI, DA-JING , LIU CHUN-QUAN : "Advances on Extraction and Analysis Method of Lutein from Marigold (Tagets erecta)", FOOD SCIENCE , vol. 26, no. 9, 15 September 2005 (2005-09-15), pages 582 - 586, XP055775377
BARZANA E; RUBIO D; SANTAMARIA R I; GARCIA-CORREA O; GARCIA F; RIDAURA SANZ V E; LÓPEZ-MUNGUÍA A: "Enzyme-Mediated Solvent Extraction of Carotenoids from Marigold Flower ( Tagetes erecta )", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 50, no. 16, 1 July 2002 (2002-07-01), pages 4491 - 4496, XP055682370, ISSN: 0021-8561, DOI: 10.1021/jf025550q
BONE ET AL., INVEST. OPHTHAMAL. VIS. SCI., vol. 34, 1993, pages 2033 - 2040
SEDDON ET AL., J.AM.MED.ASSOC., vol. 272, 1994, pages 1413 - 1420
See also references of EP 3822257A4
BARZANA E; RUBIO D; SANTAMARIA R I; GARCIA-CORREA O; GARCIA F; RIDAURA SANZ V E; LÓPEZ-MUNGUÍA A: "Enzyme-Mediated Solvent Extraction of Carotenoids from Marigold Flower ( Tagetes erecta )", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 50, no. 16, 1 July 2002 (2002-07-01), pages 4491 - 4496, XP055682370, ISSN: 0021-8561, DOI: 10.1021/jf025550q
BONE ET AL., INVEST. OPHTHAMAL. VIS. SCI., vol. 34, 1993, pages 2033 - 2040
SEDDON ET AL., J.AM.MED.ASSOC., vol. 272, 1994, pages 1413 - 1420
See also references of EP 3822257A4
Attorney, Agent or Firm:
FAITHFULAW INTERNATIONAL IP LAW OFFICE (CN)
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