Title:
METHOD OF EXTRACTING NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO/2016/026829
Kind Code:
A9
Abstract:
The method of isolating nucleic acids from a sample for further analysis by a DNA polymerase chain reaction, wherein the cells of the sample are lysed in aqueous solution using dielectric heating, which encompasses subjecting the sample to microwave electromagnetic radiation having wavelengths ranging from 11.8 to 12.6 cm, comprises the steps of providing a vessel made of microwave-transparent material with closable means and loading said vessel with a sample to be analyzed, a tablet comprising water insoluble hydrated magnesium silicate and crystalline phosphate buffer saline, and water; selecting a hollow receptacle made of microwave-reflecting material which cooperates with microwave electromagnetic radiation; placing one or more closed vessels containing said loaded mixture inside said hollow receptacle; introducing one or more said receptacles with one or more said closed vessels inside a microwave oven; and microwaving, so that the microwave electromagnetic radiation elicits a temperature rise of the mixture inside the closed vessel up to 95°C, and nucleic acids are released and a solution of nucleic acids suitable for PCR analysis is provided.
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Inventors:
WEBER WOLFGANG (DE)
WERNER CHRISTINE (DE)
NOAK NADINE (DE)
WERNER CHRISTINE (DE)
NOAK NADINE (DE)
Application Number:
PCT/EP2015/068887
Publication Date:
August 25, 2016
Filing Date:
August 18, 2015
Export Citation:
Assignee:
IFP PRIVATES INST FUER PRODUKTQUALITAET GMBH (DE)
International Classes:
C12N15/10
Attorney, Agent or Firm:
NEBENS IP (UlrichOberföhringer Str. 172, München, DE)
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