METHOD FOR EXTRACTING TOTAL MICROBIAL DNA FROM MILK

Title:

METHOD FOR EXTRACTING TOTAL MICROBIAL DNA FROM MILK

Document Type and Number:

WIPO Patent Application WO/2019/223084

Kind Code:

A1

Abstract:

Provided is a milk microbial DNA extraction method for use in direct PCR amplification, comprising: using a TE solution to treat milk, using a bead mill to fully destroy microbial cells, and using high-vacuum silicone grease to fix proteins in an intermediate layer of an aqueous phase and an organic phase so as to prevent impurities of the intermediate layer from entering the aqueous phase, and so on. The quality of the extracted DNA is intact, and the concentration and purity thereof is high.

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Inventors:

WANG JIAQI (CN)
ZHAO SHENGGUO (CN)
ZHENG NAN (CN)
ZHOU XU (CN)
LI SONGLI (CN)

Application Number:

PCT/CN2018/095679

Publication Date:

November 28, 2019

Filing Date:

July 13, 2018

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Assignee:

INST ANIMAL SCIENCES CAAS (CN)

International Classes:

C12Q1/68; C12Q1/06

Foreign References:

CN103993071A	2014-08-20
CN106497913A	2017-03-15
CN102174509A	2011-09-07
CN1544941A	2004-11-10

Attorney, Agent or Firm:

BEIJING ORIGINTELLIGENCE IP LAW FIRM (CN)

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