Title:
METHOD FOR FABRICATING CELL AGGREGATE FOR SELF-ORGANIZATION
Document Type and Number:
WIPO Patent Application WO/2015/129822
Kind Code:
A1
Abstract:
Provided is a method for forming a cell aggregate for self-organization, whereby it is possible to discover conditions necessary for fabricating a cell aggregate from numerous cells (tens of thousands to hundreds of thousands) in vitro, and to realize complex higher-order structures of a liver, kidney, or the like or interactions thereof with other organs. A method for fabricating a cell aggregate in vitro, the method including culturing a mixture of at least a total cell count of 400,000 cells of any cell and/or tissue type and 100,000 to 400,000 mesenchymal cells and forming a cell aggregate having a size of at least 1 mm. A cell aggregate fabricated by the aforementioned method. A method for fabricating a three-dimensional tissue structure, the method including causing a cell aggregate fabricated by the aforementioned method to self-organize and forming a three-dimensional tissue structure endowed with a higher-order structure. A gelled culture support, the cross-section of which on the culturing side thereof being U-shaped or V-shaped.
Inventors:
TAKEBE TAKANORI (JP)
TANIGUCHI HIDEKI (JP)
YOSHIKAWA HIROSHI (JP)
TANIGUCHI HIDEKI (JP)
YOSHIKAWA HIROSHI (JP)
Application Number:
PCT/JP2015/055695
Publication Date:
September 03, 2015
Filing Date:
February 26, 2015
Export Citation:
Assignee:
PUBLIC UNIV CORP YOKOHAMA CITY (JP)
UNIV SAITAMA NAT UNIV CORP (JP)
UNIV SAITAMA NAT UNIV CORP (JP)
International Classes:
C12N5/071; A61L27/00; C12M3/00; C12N5/0735; C12N5/077; C12N5/0775; C12N5/10
Domestic Patent References:
| WO2013047639A1 | 2013-04-04 | |||
| WO2012119074A1 | 2012-09-07 |
Other References:
TAKEBE T. ET AL.: "Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant", NAT.PROTOC., vol. 9, no. 2, 2014, pages 396 - 409, XP055166485
ZHANG M. ET AL.: "Rapid and efficient generation of neurons from human pluripotent stem cells in a multititre plate format", J.VIS.EXP., vol. 5, no. 73, 2013, pages e4335, XP055221183
ISENBERG B.C. ET AL.: "Vascular smooth muscle cell durotaxis depends on substrate stiffness gradient strength", BIOPHYS.J., vol. 9 7, no. 5, 2009, pages 1313 - 22, XP055221184
CHAWLA K. ET AL.: "Biodegradable and biocompatible synthetic saccharide-Peptide hydrogels for three-dimensional stem cell culture", BIOMACROMOLECULES, vol. 12, no. 3, 2011, pages 560 - 567, XP055221190
FISCHER R.S. ET AL.: "Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior", NAT.PROTOC., vol. 7, no. 11, 2012, pages 2056 - 66, XP055221192
OWEN S.C. ET AL.: "Design of three-dimensional biomimetic scaffolds", J.BIOMED.MATER.RES.A, vol. 94, no. 4, 2010, pages 1321 - 31, XP055221201
WANG L.S. ET AL.: "Enzymatically cross-linked gelatin-phenol hydrogels with a broader stiffness range for osteogenic differentiation of human mesenchymal stem cells", ACTA BIOMATER., vol. 8, no. 5, 2012, pages 1826 - 37, XP028476153
KUBOKI T. ET AL.: "2D-DIGE proteomic analysis of mesenchymal stem cell cultured on the elasticity-tunable hydrogels", CELL STRUCT. FUNCT., vol. 37, no. 2, 2012, pages 127 - 39, XP055357951
HIDEKI TANIGUCHI ET AL.: "Kansaibo to Bisho Kankyo no Sogo Sayo ni Motozuku Hito Sanjigen Soshiki no Jin'iteki Saikosei", MOLECULAR TARGETED THERAPY FOR CANCER, vol. 12, no. 3, 2014, pages 66 - 71, XP008184611
TAKEBE T. ET AL.: "Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell -Driven Condensation", CELL STEM CELL, vol. 16, no. 5, 2015, pages 556 - 65, XP055329771
See also references of EP 3124600A4
ZHANG M. ET AL.: "Rapid and efficient generation of neurons from human pluripotent stem cells in a multititre plate format", J.VIS.EXP., vol. 5, no. 73, 2013, pages e4335, XP055221183
ISENBERG B.C. ET AL.: "Vascular smooth muscle cell durotaxis depends on substrate stiffness gradient strength", BIOPHYS.J., vol. 9 7, no. 5, 2009, pages 1313 - 22, XP055221184
CHAWLA K. ET AL.: "Biodegradable and biocompatible synthetic saccharide-Peptide hydrogels for three-dimensional stem cell culture", BIOMACROMOLECULES, vol. 12, no. 3, 2011, pages 560 - 567, XP055221190
FISCHER R.S. ET AL.: "Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior", NAT.PROTOC., vol. 7, no. 11, 2012, pages 2056 - 66, XP055221192
OWEN S.C. ET AL.: "Design of three-dimensional biomimetic scaffolds", J.BIOMED.MATER.RES.A, vol. 94, no. 4, 2010, pages 1321 - 31, XP055221201
WANG L.S. ET AL.: "Enzymatically cross-linked gelatin-phenol hydrogels with a broader stiffness range for osteogenic differentiation of human mesenchymal stem cells", ACTA BIOMATER., vol. 8, no. 5, 2012, pages 1826 - 37, XP028476153
KUBOKI T. ET AL.: "2D-DIGE proteomic analysis of mesenchymal stem cell cultured on the elasticity-tunable hydrogels", CELL STRUCT. FUNCT., vol. 37, no. 2, 2012, pages 127 - 39, XP055357951
HIDEKI TANIGUCHI ET AL.: "Kansaibo to Bisho Kankyo no Sogo Sayo ni Motozuku Hito Sanjigen Soshiki no Jin'iteki Saikosei", MOLECULAR TARGETED THERAPY FOR CANCER, vol. 12, no. 3, 2014, pages 66 - 71, XP008184611
TAKEBE T. ET AL.: "Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell -Driven Condensation", CELL STEM CELL, vol. 16, no. 5, 2015, pages 556 - 65, XP055329771
See also references of EP 3124600A4
Attorney, Agent or Firm:
MAYAMA, Setsuko et al. (JP)
Setsuko Mayama (JP)
Setsuko Mayama (JP)
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