Title:
METHOD FOR FERMENTATIVELY PRODUCING L-GLUTAMIC ACID DERIVED FROM NITROGEN MOLECULE
Document Type and Number:
WIPO Patent Application WO/2024/048661
Kind Code:
A1
Abstract:
The purpose of the present invention is to develop a method for fermentatively producing L-glutamic acid based on a nitrogen molecule in air using nitrogen-fixing bacteria. A culture method, by which a state in which the nitrogen-fixing bacteria have enhanced nitrogen fixation ability is maintained, has been developed. The culture method is characterized by allowing an organic acid such as citric acid to coexist in a nitrogen-limited medium put in an open-system container. A method for fermentatively producing L-glutamic acid dependent on nitrogen fixation could be developed by culturing genetically modified strains, which highly express a citric acid synthase gene and a citric acid transporter gene, by means of the culture method. A method for fermentatively producing L-glutamic acid dependent on nitrogen fixation could be developed by using modified strains, which highly express a citric acid synthase gene or a 2-methylcitric acid synthase gene, in the process to reach the aforementioned step. The present invention pertains to a production method and a culture method for such microorganisms.
Inventors:
YOSHIDOME DAISUKE (JP)
ITO YUSUKE (JP)
YAMASHITA KONA (JP)
HIDAKA MAKOTO (JP)
MATSUDA SAORI (JP)
NISHIYAMA MAKOTO (JP)
ITO YUSUKE (JP)
YAMASHITA KONA (JP)
HIDAKA MAKOTO (JP)
MATSUDA SAORI (JP)
NISHIYAMA MAKOTO (JP)
Application Number:
PCT/JP2023/031570
Publication Date:
March 07, 2024
Filing Date:
August 30, 2023
Export Citation:
Assignee:
KIKKOMAN CORP (JP)
UNIV TOKYO (JP)
UNIV TOKYO (JP)
International Classes:
C12P1/04; C12N1/00; C12N1/20; C12N1/21; C12N15/52; C12N15/54; C12P13/14
Domestic Patent References:
| WO1998018929A1 | 1998-05-07 |
Foreign References:
| JPS63119688A | 1988-05-24 | |||
| JP2009254323A | 2009-11-05 | |||
| JP2000189175A | 2000-07-11 |
Other References:
YOSHIDOME, DAISUKE ET AL.: "L-Glutamate production from aerial nitrogen using nitrogen-fixing bacteria", PROCEEDINGS (ONLINE) OF THE ANNUAL MEETING OF JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY 2023, 2B04-01, 5 March 2023 (2023-03-05), pages 2B04-01
Attorney, Agent or Firm:
AOKI, Atsushi et al. (JP)
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