Title:
METHOD FOR FORMULATING NON-ENVELOPED VIRAL VECTOR PARTICLES BY CHANGE IN pH
Document Type and Number:
WIPO Patent Application WO/2022/045055
Kind Code:
A1
Abstract:
The present invention provides a method that is for obtaining an AAV formulation from a sample containing AAV vector particles and empty capsids of AAV, and that comprises: (a) a step for applying the sample containing AAV vector particles and empty capsids of AAV to an anionic exchange carrier equilibrated with a buffer so as to cause the AAV vector particles to be bound to the carrier; (b) a step for applying a buffer to the carrier after step (a) so that non-bound substances are eluted from the carrier; (c) a step for applying a buffer having a pH lower than that of the buffer used to equilibrate the carrier to the carrier after step (b) so that the AAV vector particles are eluted from the carrier; and (d) a step for collecting an eluate obtained from the carrier in step (c) to obtain an AAV formulation.
Inventors:
INOSE HARUKO (JP)
CHONO HIDETO (JP)
TSUKIHARA TAKAHISA (JP)
KAMADA YUKO (JP)
ABE KENSUKE (JP)
NISHIE TOSHIKAZU (JP)
TANAKA YOSHINORI (JP)
CHONO HIDETO (JP)
TSUKIHARA TAKAHISA (JP)
KAMADA YUKO (JP)
ABE KENSUKE (JP)
NISHIE TOSHIKAZU (JP)
TANAKA YOSHINORI (JP)
Application Number:
PCT/JP2021/030774
Publication Date:
March 03, 2022
Filing Date:
August 23, 2021
Export Citation:
Assignee:
TAKARA BIO INC (JP)
International Classes:
C12N7/02
Foreign References:
| JP2018507707A | 2018-03-22 | |||
| JP2007117003A | 2007-05-17 | |||
| JP2006528886A | 2006-12-28 | |||
| JP2019509745A | 2019-04-11 |
Attorney, Agent or Firm:
YAMAO, Norihito et al. (JP)
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