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Patent Searching and Data


Title:
METHOD FOR FORMULATING NON-ENVELOPED VIRAL VECTOR PARTICLES BY CHANGE IN pH
Document Type and Number:
WIPO Patent Application WO/2022/045055
Kind Code:
A1
Abstract:
The present invention provides a method that is for obtaining an AAV formulation from a sample containing AAV vector particles and empty capsids of AAV, and that comprises: (a) a step for applying the sample containing AAV vector particles and empty capsids of AAV to an anionic exchange carrier equilibrated with a buffer so as to cause the AAV vector particles to be bound to the carrier; (b) a step for applying a buffer to the carrier after step (a) so that non-bound substances are eluted from the carrier; (c) a step for applying a buffer having a pH lower than that of the buffer used to equilibrate the carrier to the carrier after step (b) so that the AAV vector particles are eluted from the carrier; and (d) a step for collecting an eluate obtained from the carrier in step (c) to obtain an AAV formulation.

Inventors:
INOSE HARUKO (JP)
CHONO HIDETO (JP)
TSUKIHARA TAKAHISA (JP)
KAMADA YUKO (JP)
ABE KENSUKE (JP)
NISHIE TOSHIKAZU (JP)
TANAKA YOSHINORI (JP)
Application Number:
PCT/JP2021/030774
Publication Date:
March 03, 2022
Filing Date:
August 23, 2021
Export Citation:
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Assignee:
TAKARA BIO INC (JP)
International Classes:
C12N7/02
Foreign References:
JP2018507707A2018-03-22
JP2007117003A2007-05-17
JP2006528886A2006-12-28
JP2019509745A2019-04-11
Attorney, Agent or Firm:
YAMAO, Norihito et al. (JP)
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