Title:
A METHOD FOR IDENTIFICATION, ISOLATION AND PRODUCTION OF ANTIGENS TO A SPECIFIC PATHOGEN
Document Type and Number:
WIPO Patent Application WO2002059148
Kind Code:
A9
Abstract:
Described is a method for identification, isolation and production of hyperimmune serum-reactive antigens from a specific pathogen, a tumor, an allergen or a tissue or host prone to autoimmunity, said antigens being suited for use in a vaccine for a given type of animal or for humans, which is characterized by the following steps: - providing an antibody preparation from a plasma pool of said given type of animal or from a human plasma pool or individual sera with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, - providing at least one expression library of said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, - screening said at least one expression library with said antibody preparation, - identifying antigens which bind in said screening to antibodies in said antibody preparation, - screening the identified antigens with individual antibody preparations from individual sera from individuals with antibodies against said specific pathogen, tumor, allergen or tissue or host prone to auto-immunity, - identifying the hyperimmune serum-reactive antigen portion of said identified antigens and which hyperimmune serum-reactive antigens bind to a relevant portion of said individual antibody preparations from said individual sera and - optionally isolating said hyperimmune serum-reactive antigens and producing said hyperimmune serum-reactive antigens by chemical or recombinant methods.
Inventors:
MEINKE ANDREAS (AT)
NAGY ESZTER (AT)
VON AHSEN UWE (AT)
KLADE CHRISTOPH (AT)
HENICS TAMAS (AT)
ZAUNER WOLFGANG (AT)
MINH DUC BUI (AT)
VYTVYTSKA ORESTA (AT)
ETZ HILDEGARD (AT)
DRYLA AGNIESZKA (AT)
WEICHHART THOMAS (AT)
HAFNER MARTIN (AT)
TEMPELMAIER BRIGITTE (AT)
FRASER CLAIRE M (US)
GILL STEVEN (US)
NAGY ESZTER (AT)
VON AHSEN UWE (AT)
KLADE CHRISTOPH (AT)
HENICS TAMAS (AT)
ZAUNER WOLFGANG (AT)
MINH DUC BUI (AT)
VYTVYTSKA ORESTA (AT)
ETZ HILDEGARD (AT)
DRYLA AGNIESZKA (AT)
WEICHHART THOMAS (AT)
HAFNER MARTIN (AT)
TEMPELMAIER BRIGITTE (AT)
FRASER CLAIRE M (US)
GILL STEVEN (US)
Application Number:
PCT/EP2002/000546
Publication Date:
October 31, 2002
Filing Date:
January 21, 2002
Export Citation:
Assignee:
CISTEM BIOTECHNOLOGIES GMBH (AT)
MEINKE ANDREAS (AT)
NAGY ESZTER (AT)
AHSEN UWE (AT)
KLADE CHRISTOPH (AT)
HENICS TAMAS (AT)
ZAUNER WOLFGANG (AT)
MINH DUC BUI (AT)
VYTVYTSKA ORESTA (AT)
ETZ HILDEGARD (AT)
DRYLA AGNIESZKA (AT)
WEICHHART THOMAS (AT)
HAFNER MARTIN (AT)
TEMPELMAIER BRIGITTE (AT)
FRASER CLAIRE M (US)
GILL STEVEN (US)
MEINKE ANDREAS (AT)
NAGY ESZTER (AT)
AHSEN UWE (AT)
KLADE CHRISTOPH (AT)
HENICS TAMAS (AT)
ZAUNER WOLFGANG (AT)
MINH DUC BUI (AT)
VYTVYTSKA ORESTA (AT)
ETZ HILDEGARD (AT)
DRYLA AGNIESZKA (AT)
WEICHHART THOMAS (AT)
HAFNER MARTIN (AT)
TEMPELMAIER BRIGITTE (AT)
FRASER CLAIRE M (US)
GILL STEVEN (US)
International Classes:
G01N33/50; A61K39/00; A61K39/39; A61K39/395; A61P31/10; B64C9/02; C07K14/195; C07K14/31; C07K16/12; C12N15/02; C12N15/09; C12N15/10; C12P21/02; C12P21/08; C12Q1/68; C12Q1/6806; C40B40/02; G01N33/15; G01N33/53; (IPC1-7): C07K14/195
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