Title:
METHOD FOR IDENTIFYING TOLERANCE IN GRAFT RECIPIENTS
Document Type and Number:
WIPO Patent Application WO2003005972
Kind Code:
A3
Abstract:
Provided is a method of identifying tolerance in allograft recipients. It has been found that higher levels of circulating plasmacytoid DC (pDC2 cells), as compared to monocytoid (pDC1) cells (pDC2/pDC1) correlates with transplant tolerance. In one embodiment, the method includes determining the relative amounts of HLA-DR<+>lin<-> (CD3<->CD14<->CD19<->CD20<->)CD123<+>CD11c<->(pDC2) cells and HLA-DR<+>lin<-> CD123CD11c<+>(pDC1) cells in peripheral blood. The pDC1/pDC2 either can be determined from direct analysis of cells in a peripheral blood sample, or DC1 and DC2 cells can be cultured from a peripheral blood sample and the numbers of pDC1 and pDC2 cells in the peripheral blood sample can be estimated from the growth of the DC1 and DC2 cells in culture.
Inventors:
THOMSON ANGUS W
Application Number:
PCT/US2002/022636
Publication Date:
November 06, 2003
Filing Date:
July 15, 2002
Export Citation:
Assignee:
UNIV PITTSBURGH (US)
International Classes:
G01N33/49; G01N33/50; G01N33/569; (IPC1-7): G01N33/48; G01N33/549
Other References:
ARPINATI ET AL.: "Donor DC1:DC2 ratio correlates with post transplant immunologic events in allogeneic PBSC transplantation", BLOOD, vol. 96, no. 11, PART 1, November 2000 (2000-11-01), pages 397A, XP002963551
REDDY ET AL.: "Circulating dendritic cells (DC1 and DC2) at engraftment correlate with relapse and survival after allogeneic stem cell transplantation", BLOOD, vol. 98, no. 11, PART 1, November 2001 (2001-11-01), pages 415A, XP002963552
STOCHHI ET AL.: "Dendritic cells (DC1 and DC2) subsets detection in bone marrow and G-CSF-primed peripheral blood harvests from allogeneic stem cell donors", BLOOD, vol. 96, no. 11, PART 2, November 2000 (2000-11-01), pages 307B, XP002963553
TZOLAS ET AL.: "Type 1 dendritic cells (DC1) in allogeneic donor peripheral blood stem cell product are associated with an increase in acute graft-versus-host-disease", BLOOD, vol. 98, no. 11, PART 1, November 2001 (2001-11-01), pages 203A - 204A, XP002963554
REDDY ET AL.: "Circulating dendritic cells (DC1 and DC2) at engraftment correlate with relapse and survival after allogeneic stem cell transplantation", BLOOD, vol. 98, no. 11, PART 1, November 2001 (2001-11-01), pages 415A, XP002963552
STOCHHI ET AL.: "Dendritic cells (DC1 and DC2) subsets detection in bone marrow and G-CSF-primed peripheral blood harvests from allogeneic stem cell donors", BLOOD, vol. 96, no. 11, PART 2, November 2000 (2000-11-01), pages 307B, XP002963553
TZOLAS ET AL.: "Type 1 dendritic cells (DC1) in allogeneic donor peripheral blood stem cell product are associated with an increase in acute graft-versus-host-disease", BLOOD, vol. 98, no. 11, PART 1, November 2001 (2001-11-01), pages 203A - 204A, XP002963554
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