Title:
METHOD FOR IMPROVING EXPRESSION OF LIPASE BY GLYCOSYLATION MODIFICATION, MUTANT ENZYMES AND APPLICATION OF MUTANT ENZYMES
Document Type and Number:
WIPO Patent Application WO/2016/169306
Kind Code:
A1
Abstract:
Provided are a method for improving the expression of lipase by glycosylation modification, mutant enzymes and an application of the mutant enzymes, specifically comprising: performing N-glycosylation mutation on a propeptide sequence of rhizopus oryzaelipase, and respectively modifying SAS and/or NT amino acids into N-glycosylation loci NGT and/or NLT; compared with the extracelluar protein concentration of proROL which is not subjected to glycosylation, extracelluar protein concentrations of obtained mutant enzymes proROLA, proROLB and proROLAB are separately improved by 211 percent, 188 percent and 233 percent; and when a fermentation tank is adopted for culturing, enzyme activities separately reach 8210U·mL-1, 8457U·mL-1 and 9366U·mL-1, and the extracellular enzyme activity of unmutated proROL is almost zero.
Inventors:
YU XIAO WEI (CN)
XU YAN (CN)
XU YAN (CN)
Application Number:
PCT/CN2016/070236
Publication Date:
October 27, 2016
Filing Date:
January 06, 2016
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N9/20; C12N1/19; C12N15/81; C12R1/84; C12R1/845
Foreign References:
| CN104762277A | 2015-07-08 | |||
| CN101857856A | 2010-10-13 | |||
| CN102363786A | 2012-02-29 |
Other References:
DATABASE GenBank [O] 12 February 2004 (2004-02-12), XP055323713, Database accession no. AAF32408.1
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO.,LTD. (CN)
哈尔滨市阳光惠远知识产权代理有限公司 (CN)
哈尔滨市阳光惠远知识产权代理有限公司 (CN)
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