Title:
METHOD FOR IMPROVING THERMOSTABILITY OF PHYTASE, MUTANT AND USE
Document Type and Number:
WIPO Patent Application WO/2024/001184
Kind Code:
A1
Abstract:
A mutant of phytase APPAmut4 is provided. Compared with parents, the mutant has enhanced phytase thermostability, and an optimal mutant APPAmut9 can still retain about 70% of activity after being treated at 100 ℃ for 5 min. The provided phytase mutant can be well used in the industries of energy, food and feed and has broad application prospects.
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Inventors:
HUANG HUOQING (CN)
TU TAO (CN)
WANG QIAN (CN)
YAO BIN (CN)
LUO HUIYING (CN)
BAI YINGGUO (CN)
WANG YUAN (CN)
SU XIAOYUN (CN)
WANG YARU (CN)
ZHANG JIE (CN)
QIN XING (CN)
WANG XIAOLU (CN)
ZHANG HONGLIAN (CN)
YU HUIMIN (CN)
TU TAO (CN)
WANG QIAN (CN)
YAO BIN (CN)
LUO HUIYING (CN)
BAI YINGGUO (CN)
WANG YUAN (CN)
SU XIAOYUN (CN)
WANG YARU (CN)
ZHANG JIE (CN)
QIN XING (CN)
WANG XIAOLU (CN)
ZHANG HONGLIAN (CN)
YU HUIMIN (CN)
Application Number:
PCT/CN2023/073753
Publication Date:
January 04, 2024
Filing Date:
January 30, 2023
Export Citation:
Assignee:
INST OF ANIMAL SCIENCE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES (CN)
International Classes:
C12N1/19; C12N9/16; C12N15/55; C12N15/81; C12R1/84
Domestic Patent References:
| WO2017215218A1 | 2017-12-21 | |||
| WO2007128160A1 | 2007-11-15 |
Foreign References:
| CN114807087A | 2022-07-29 | |||
| CN102597227A | 2012-07-18 | |||
| CN106434595A | 2017-02-22 | |||
| CN101426907A | 2009-05-06 |
Other References:
CHEN HUI, WANG HONG-NING, YANG WAN-SHEN, ZHAO HAI-XIA, WU QI, SHAN ZHI: "Site-directed Mutagenesis Improves the Thermostability and Specific-activity of Phytase Mutated by F43Y and I354M, L358F", ZHONGGUO SHENGWU HUAXUE YU FENZI SHENGWU XUEBAO - CHINESE JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, BEIJING DAXUE YIXUEBU, CN, vol. 21, no. 4, 15 August 2005 (2005-08-15), CN , pages 516 - 520, XP009551566, ISSN: 1007-7626, DOI: 10.13865/j.cnki.cjbmb.2005.04.015
DATABASE Protein 14 July 2016 (2016-07-14), ANONYMOUS : "phytase [Yersinia intermedia] ", XP093122554, Database accession no. ABI95370.1
DATABASE Protein 14 July 2016 (2016-07-14), ANONYMOUS : "phytase [Yersinia intermedia] ", XP093122554, Database accession no. ABI95370.1
Attorney, Agent or Firm:
FASIN IP & PARTNERS (CN)
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