Title:
METHOD FOR IN VITRO EXPANSION OF CD8+T CELLS AND CELL SUBSETS THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/133598
Kind Code:
A1
Abstract:
Disclosed is a method for the rapid in vitro expansion of CD8+T cells and functional cell subsets thereof. A TLR1/2 agonist, a TLR2/6 agonist, a TLR5 agonist, or a combination of said agonists, is added to a conventional in vitro culture system for culturing expanded CD8+T cells; recombinant cytokines IL-2, IL-7, and IL-15, and anti-human CD3 antibody and anti-human CD28 antibody-coated magnetic beads can further be added to implement joint and continuous stimulation.
Inventors:
XU JIANQING (CN)
ZHANG XIAOYAN (CN)
QIU CHENLI (CN)
ZHANG XIAOYAN (CN)
QIU CHENLI (CN)
Application Number:
PCT/CN2017/116342
Publication Date:
July 26, 2018
Filing Date:
December 15, 2017
Export Citation:
Assignee:
SHANGHAI PUBLIC HEALTH CLINICAL CT (CN)
International Classes:
C12N5/0783
Domestic Patent References:
| WO2009062001A1 | 2009-05-14 |
Foreign References:
| CN105451766A | 2016-03-30 |
Other References:
WANG, YUNFEI ET AL.: "CD 3/ CD 28 Immunomagnetic Bead Method for Ex Vivo Expansion of T Lymphocytes of Peripheral Blood", CHINESE JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY, vol. 30, no. 10, 31 October 2014 (2014-10-31), pages 1090 - 1092
Attorney, Agent or Firm:
CHINA PATENT AGENT (HK)LTD. (CN)
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