METHOD FOR INCREASING YIELD OR AMOUNT OF COLLAGEN THROUGH PH TITRATION

Title:

METHOD FOR INCREASING YIELD OR AMOUNT OF COLLAGEN THROUGH PH TITRATION

Document Type and Number:

WIPO Patent Application WO/2023/282554

Kind Code:

A1

Abstract:

The present invention relates to a method for increasing the yield or amount of collagen through pH titration and, more specifically, to a method for increasing the yield or amount of collagen through stepwise pH titration. In a collagen preparation method of the present invention, unlike in a conventional preparation method in which single titration is performed at a pH of an arbitrary value selected from 6-8, stepwise titration is performed in a pH range of 4-7, so that collagen cohesiveness is increased to facilitate the separation of a solvent and a solute, thereby ensuring the maximum yield of collagen, and the protein chain structure of the solute (collagen) is made to be more robust to enable the amount of collagen to be increased, and thus collagen according to the present invention is expected to have high utility in various fields such as that of 3D printing materials, regenerative medical materials and xeno-organ-related medical devices.

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Inventors:

LEE JONG HYUCK (KR)
HA JAE YUN (KR)
LEE DONG HYUCK (KR)
KIM BYUNG JUN (KR)
LEE TAE WON (KR)
YOOK IL SOO (KR)

Application Number:

PCT/KR2022/009583

Publication Date:

January 12, 2023

Filing Date:

July 04, 2022

Export Citation:

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Assignee:

MEDICA KOREA CO LTD (KR)

International Classes:

A61L27/36; A61L27/24

Foreign References:

KR102155509B1	2020-09-14
KR101871395B1	2018-06-26
KR101531479B1	2015-06-26
KR101760890B1	2017-07-24

Other References:

SHI LINFAN, HAOHAO TIAN, YANXIA WANG, GENGXIN HAO, JUN CHEN, WUYIN WENG : "Effect of pH on properties of golden pompano skin collagen-based fibril gels by self-assembly in vitro", J. SCI. FOOD AGRIC., vol. 100, no. 13, pages 4801 - 4807, XP093022423, DOI: 10.1002/jsfa.10539
LI, Y. ; ASADI, A. ; MONROE, M.R. ; DOUGLAS, E.P.: "pH effects on collagen fibrillogenesis in vitro: Electrostatic interactions and phosphate binding", MATERIALS SCIENCE AND ENGINEERING C, ELSEVIER SCIENCE S.A., CH, vol. 29, no. 5, 1 June 2009 (2009-06-01), CH , pages 1643 - 1649, XP026107131, ISSN: 0928-4931, DOI: 10.1016/j.msec.2009.01.001

Attorney, Agent or Firm:

ISIS IP LAW LLC (KR)

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