Title:
METHOD FOR INCREASING YIELD OF EICOSAPENTAENOIC ACID IN SCHIZOCHYTRIUM SP.
Document Type and Number:
WIPO Patent Application WO/2022/198990
Kind Code:
A1
Abstract:
A method for increasing the yield of eicosapentaenoic acid in Schizochytrium sp., the method comprising: inoculating Schizochytrium sp. ATCC 20888 into a fermentation culture medium, fermenting same under an aerobic condition, changing the temperature when fermenting is performed to the middle of a logarithmic phase, continuing fermenting same, and controlling the dissolved oxygen (DO) value to be 2%-10% after changing the temperature, wherein changing the temperature increases the initial fermentation temperature to 32°C-37°C from 25°C-30°C. EPA is produced by means of fermenting with Schizochytrium sp. ATCC 20888, the dry weight of thalli in the obtained fermentation liquor reaches 66.15 g/L, the yield of oil is 9.97 g/L, and EPA accounts for 13.33% of fatty acid.
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Inventors:
WANG YONGHUA (CN)
MO SHUXIN (CN)
XIAO ZHENG (CN)
LAN DONGMING (CN)
LEI CHANGMEI (CN)
MO SHUXIN (CN)
XIAO ZHENG (CN)
LAN DONGMING (CN)
LEI CHANGMEI (CN)
Application Number:
PCT/CN2021/124344
Publication Date:
September 29, 2022
Filing Date:
October 18, 2021
Export Citation:
Assignee:
UNIV SOUTH CHINA TECH (CN)
YHSN TECH LTD (CN)
YHSN TECH LTD (CN)
International Classes:
C12P7/64; C12N1/14; C12R1/645
Foreign References:
| CN105018539A | 2015-11-04 | |||
| CN113005154A | 2021-06-22 | |||
| CN103882072A | 2014-06-25 | |||
| CN1648233A | 2005-08-03 | |||
| CN108707630A | 2018-10-26 |
Attorney, Agent or Firm:
GUANGZHOU HUAXUE INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
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