Title:
METHOD OF INDUCING THE DIFFERENTIATION OF STEM CELLS INTO MYOCARDIAL CELLS
Document Type and Number:
WIPO Patent Application WO/2005/033298
Kind Code:
A1
Abstract:
It is intended to provide a method of inducing the differentiation of stem cells into myocardial cells characterized in that the induction of the differentiation of stem cells into myocardial cells is carried out by culturing the stem cells in the presence of a substance inhibiting the BMP signal transfer to thereby efficiently and selectively induce the differentiation into myocardial cells.
Inventors:
FUKUDA KEIICHI (JP)
YUASA SHINSUKE (JP)
OKANO HIDEYUKI (JP)
SHIMAZAKI TAKUYA (JP)
KOSHIMIZU UICHI (JP)
TANAKA TOMOFUMI (JP)
SUGIMURA KEIJIRO (JP)
YUASA SHINSUKE (JP)
OKANO HIDEYUKI (JP)
SHIMAZAKI TAKUYA (JP)
KOSHIMIZU UICHI (JP)
TANAKA TOMOFUMI (JP)
SUGIMURA KEIJIRO (JP)
Application Number:
PCT/JP2004/014598
Publication Date:
April 14, 2005
Filing Date:
October 04, 2004
Export Citation:
Assignee:
FUKUDA KEIICHI (JP)
YUASA SHINSUKE (JP)
OKANO HIDEYUKI (JP)
SHIMAZAKI TAKUYA (JP)
KOSHIMIZU UICHI (JP)
TANAKA TOMOFUMI (JP)
SUGIMURA KEIJIRO (JP)
YUASA SHINSUKE (JP)
OKANO HIDEYUKI (JP)
SHIMAZAKI TAKUYA (JP)
KOSHIMIZU UICHI (JP)
TANAKA TOMOFUMI (JP)
SUGIMURA KEIJIRO (JP)
International Classes:
C12N5/077; A61K35/12; A61K35/545; A61P9/04; C12N5/07; C12N5/071; C12N5/0735; C12N5/074; C12N5/0789; C12N15/09; (IPC1-7): C12N5/06; C12N5/08
Other References:
MONZEN K. ET AL.: "Bone morphogenetic proteins induce cardiomyocyte differentiation through the mitogen-activated protein kinase kinase kinase TAK1 and cardiac transcription factors Csx/Nkx-2.5 and GATA-4", MOL. CELL. BIOL., vol. 19, no. 10, 1999, pages 7096 - 7105, XP002958522
KAWAI T. ET AL.: "Efficient cardiomyogenic differentiation of embryonic stem cell by fibroblast growth factor 2 and bone morphogenetic protein 2", CIRC. J., vol. 68, no. 7, July 2004 (2004-07-01), pages 691 - 702, XP002983463
SMITH; HARLAND, CELL, vol. 70, 1992, pages 829
SASAI ET AL., CELL, vol. 79, 1994, pages 779
RE'EM-KALMA ET AL., PROC. NATL. ACAD. SCI. USA, vol. 92, 1995, pages 12141
ZIMMERMAN ET AL., CELL, vol. 86, 1996, pages 599
PICCOLO ET AL., CELL, vol. 86, 1996, pages 589
GRATSCH; O'SHEA, DEV. BIOL., vol. 245, 2002, pages 83
SCHULTHEISS ET AL., GENES DEV., vol. 11, 1997, pages 451
SPARROW ET AL., MECH. DEV., vol. 71, 1998, pages 151
MONZEN ET AL., MOL. CELL. BIOL., vol. 19, 1999, pages 7096
KLUG ET AL., J. CLIN. INVEST., vol. 98, 1996, pages 216
KAWAI T. ET AL.: "Efficient cardiomyogenic differentiation of embryonic stem cell by fibroblast growth factor 2 and bone morphogenetic protein 2", CIRC. J., vol. 68, no. 7, July 2004 (2004-07-01), pages 691 - 702, XP002983463
SMITH; HARLAND, CELL, vol. 70, 1992, pages 829
SASAI ET AL., CELL, vol. 79, 1994, pages 779
RE'EM-KALMA ET AL., PROC. NATL. ACAD. SCI. USA, vol. 92, 1995, pages 12141
ZIMMERMAN ET AL., CELL, vol. 86, 1996, pages 599
PICCOLO ET AL., CELL, vol. 86, 1996, pages 589
GRATSCH; O'SHEA, DEV. BIOL., vol. 245, 2002, pages 83
SCHULTHEISS ET AL., GENES DEV., vol. 11, 1997, pages 451
SPARROW ET AL., MECH. DEV., vol. 71, 1998, pages 151
MONZEN ET AL., MOL. CELL. BIOL., vol. 19, 1999, pages 7096
KLUG ET AL., J. CLIN. INVEST., vol. 98, 1996, pages 216
Attorney, Agent or Firm:
Shamoto, Ichio (Section 206 New Ohtemachi Bldg., 2-1,
Ohtemachi 2-chom, Chiyoda-ku Tokyo, JP)
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