Title:
METHOD FOR ISOLATING, MAINTAINING, PROLIFERATING, AND DIFFERENTIATING MONOCLONAL CELLS DERIVED FROM HUMAN SALIVARY GLAND EPITHELIAL STEM CELLS OR PROGENITOR CELLS AND PRODUCTION METHOD FOR EXTRACELLULAR VESICLES FOR TREATING SALIVARY GLAND DISEASES
Document Type and Number:
WIPO Patent Application WO/2023/090928
Kind Code:
A1
Abstract:
The present invention relates to a method for isolating, maintaining, proliferating and differentiating monoclonal cells derived from human salivary gland epithelial stem cells or progenitor cells, and a production method for extracellular vesicles for treating salivary gland diseases. More specifically, conditions for isolating monoclonal salivary gland epithelial cells through an optimized subfractionation culture method from a small amount of major salivary gland or minor salivary gland tissue obtained during surgery or biopsy processes have been established, and culture medium conditions for enabling long-term culture of salivary gland epithelial cells or progenitor cells in 2D have been established. Such high-purity monoclonal cells derived from human salivary gland epithelial stem cells or precursor cells are expected to be utilized not only as cell therapy products for fundamental treatment of salivary gland dysfunction and as candidates for extracellular vesicles, but also widely throughout salivary gland studies such as disease modeling, pathology research, drug screening, toxicity evaluation, and genetic manipulation.
Inventors:
LIM JAE-YOL (KR)
YOON YEO-JUN (KR)
HONG YONGPYO (KR)
JEONG YE-JIN (KR)
YOON YEO-JUN (KR)
HONG YONGPYO (KR)
JEONG YE-JIN (KR)
Application Number:
PCT/KR2022/018276
Publication Date:
May 25, 2023
Filing Date:
November 18, 2022
Export Citation:
Assignee:
UNIV YONSEI IACF (KR)
International Classes:
C12N5/071
Foreign References:
| KR20190007701A | 2019-01-23 | |||
| KR20190024942A | 2019-03-08 |
Other References:
KIM, K. et al. A Rho Kinase (ROCK) Inhibitor, Y-27632, Inhibits the Dissociation-Induced Cell Death of Salivary Gland Stem Cells. Molecules. 2021, vol. 26, document 2658, pp. 1-12.
RAHMAN ZULAIHA A.: "The development of in vitro models of human salivary glands", PHD THESIS, PROQUEST DISSERTATIONS PUBLISHING, THE UNIVERSITY OF SHEFFIELD, 1 December 2018 (2018-12-01), The University of Sheffield, XP093068048, [retrieved on 20230727]
NAMBOODIRIPAD P.C. ANILA: "A review: Immunological markers for malignant salivary gland tumors", JOURNAL OF ORAL BIOLOGY AND CRANIOFACIAL RESEARCH, vol. 4, no. 2, 1 May 2014 (2014-05-01), pages 127 - 134, XP093068043, ISSN: 2212-4268, DOI: 10.1016/j.jobcr.2014.05.003
SCHOCK ELIZABETH N., LABONNE CAROLE: "Sorting Sox: Diverse Roles for Sox Transcription Factors During Neural Crest and Craniofacial Development", FRONTIERS IN PHYSIOLOGY, vol. 11, pages 606889, XP093068041, DOI: 10.3389/fphys.2020.606889
RAHMAN ZULAIHA A.: "The development of in vitro models of human salivary glands", PHD THESIS, PROQUEST DISSERTATIONS PUBLISHING, THE UNIVERSITY OF SHEFFIELD, 1 December 2018 (2018-12-01), The University of Sheffield, XP093068048, [retrieved on 20230727]
NAMBOODIRIPAD P.C. ANILA: "A review: Immunological markers for malignant salivary gland tumors", JOURNAL OF ORAL BIOLOGY AND CRANIOFACIAL RESEARCH, vol. 4, no. 2, 1 May 2014 (2014-05-01), pages 127 - 134, XP093068043, ISSN: 2212-4268, DOI: 10.1016/j.jobcr.2014.05.003
SCHOCK ELIZABETH N., LABONNE CAROLE: "Sorting Sox: Diverse Roles for Sox Transcription Factors During Neural Crest and Craniofacial Development", FRONTIERS IN PHYSIOLOGY, vol. 11, pages 606889, XP093068041, DOI: 10.3389/fphys.2020.606889
Attorney, Agent or Firm:
TAEBAEK INTELLECTUAL PROPERTY LAW FIRM (KR)
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