METHOD AND KIT FOR PROMOTING EXPRESSION OF TARGET PROTEIN IN MAMMALIAN CELLS, AND UTILIZATION THEREOF

WIPO Patent Application WO/2022/030094

A1

According to the present invention, the expression of a target protein is promoted in mammalian cells by co-introducing a polynucleotide containing an expression cassette of the target protein, a polynucleotide containing IR/MAR and a polynucleotide promoting the expression of the target protein, each as a separate polynucleotide, into the mammalian cells.

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SHIMIZU NORIAKI (JP)
KANEKO KANAME (JP)
OKA DAIKI (JP)

PCT/JP2021/021504

February 10, 2022

June 07, 2021

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UNIV HIROSHIMA (JP)
MEIJI SEIKA PHARMA CO LTD (JP)

C12N5/10; C12N15/85; C12P21/00

JP2016127817A	2016-07-14
JP2003245083A	2003-09-02

OKADA NAOYA, SHIMIZU NORIAKI: "Dissection of the Beta-Globin Replication-Initiation Region Reveals Specific Requirements for Replicator Elements during Gene Amplification", PLOS ONE, vol. 8, no. 10, 4 October 2013 (2013-10-04), pages e77350, XP055905480, DOI: 10.1371/journal.pone.0077350
FUKUMA MIKI, GANMYO YUTO, MIURA OSAMU, OHYAMA TAKASHI, SHIMIZU NORIAKI: "Cloning and Characterization of a Human Genomic Sequence that Alleviates Repeat-Induced Gene Silencing", PLOS ONE, vol. 11, no. 4, 14 April 2016 (2016-04-14), pages e0153338, XP055905482, DOI: 10.1371/journal.pone.0153338

HARAKENZO WORLD PATENT & TRADEMARK (JP)

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