Title:
METHOD FOR MANUFACTURING MUTANT STRAIN HAVING HIGH DEINOXANTHIN PRODUCTION ABILITY AND METHOD FOR MASS-PRODUCING DEINOXANTHIN BY USING TEMPERATURE CONTROL
Document Type and Number:
WIPO Patent Application WO/2021/256703
Kind Code:
A1
Abstract:
The present invention relates to a method for manufacturing a mutant strain having high deinoxanthin production ability, and a method for mass-producing deinoxanthin by using temperature control. Although being manufactured by applying a metabolic engineering method, a Deinococcus radiodurans mutant strain having high deinoxanthin production ability, manufactured by the method of the present invention, does not have an artificially introduced antibiotic resistance gene. The present invention can increase the production of deinoxanthin by regulating the expression of messenger RNA (mRNA) of DR0862 and DR1475 genes with the control of culture temperatures, without using an inducer such as IPTG, in the Deinococcus sp. mutant strain having both DR0862 crtB and DR1475 dxs genes introduced thereinto and exhibiting high deinoxanthin production ability. Thus, the present invention can be advantageously used for the mass production of deinoxanthin having high antioxidant activity through a very economical method.
Inventors:
CHOI YONG JUN (KR)
JEONG SUN WOOK (KR)
JEONG SUN WOOK (KR)
Application Number:
PCT/KR2021/005715
Publication Date:
December 23, 2021
Filing Date:
May 07, 2021
Export Citation:
Assignee:
UNIV SEOUL IND COOP FOUND (KR)
International Classes:
C12N15/74; C12P23/00
Foreign References:
| KR101961564B1 | 2019-03-25 | |||
| KR20160062169A | 2016-06-01 | |||
| KR101835852B1 | 2018-04-19 | |||
| CN104152389A | 2014-11-19 | |||
| JP2015502169A | 2015-01-22 |
Attorney, Agent or Firm:
LEE, Won Hee (KR)
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