Title:
METHOD FOR MANUFACTURING FAT/OIL USING YEAST BELONGING TO GENUS LIPOMYCES
Document Type and Number:
WIPO Patent Application WO/2023/084841
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for manufacturing a substitute for palm oil that can be performed on a mass scale at a low cost. The method for manufacturing a fat/oil of the present invention comprises: step A for culturing a yeast belonging to the genus Lipomyces; step B for disrupting the yeast belonging to the genus Lipomyces to give an emulsion; step C for separating the emulsion into a residue and a liquid by passing through a nonwoven or woven fabric; step D for dissolving the fat/oil in the residue in a solvent; and step E for collecting the fat/oil by volatilizing the solvent. The solvent is a mixed solvent consisting of n-hexane and ethanol at a mixing ratio of 2:1 to 5:1. Preferably, the nonwoven or woven fabric has an average pore size of 0.5-10 μm inclusive and an air permeability of from 2 cm3/(cm2∙s) to 45 cm3/(cm2∙s).
Inventors:
MATSUYAMA HIDETO
KUMAGAI KAZUO
TAKI HIROYA
MINE KENTARO
KUMAGAI KAZUO
TAKI HIROYA
MINE KENTARO
Application Number:
PCT/JP2022/026714
Publication Date:
May 19, 2023
Filing Date:
July 05, 2022
Export Citation:
Assignee:
NISSIN FOODS HOLDINGS CO LTD (JP)
UNIV KOBE NAT UNIV CORP (JP)
UNIV KOBE NAT UNIV CORP (JP)
International Classes:
B01D29/01; C12P7/64; B01D29/11; B01D35/02
Domestic Patent References:
| WO2020175310A1 | 2020-09-03 |
Foreign References:
| JP2019146543A | 2019-09-05 | |||
| JPH0799932A | 1995-04-18 | |||
| CN104531342A | 2015-04-22 | |||
| JP2018000190A | 2018-01-11 |
Other References:
KYLE V. PROBST; MICHAEL D. WALES; MARY E. REZAC; PRAVEEN V. VADLANI: "Evaluation of green solvents: Oil extraction from oleaginous yeast Lipomyces starkeyi using cyclopentyl methyl ether (CPME)", BIOTECHNOLOGY PROGRESS, AMERICAN CHEMICAL SOCIETY, HOBOKEN, USA, vol. 33, no. 4, 16 April 2017 (2017-04-16), Hoboken, USA, pages 1096 - 1103, XP072297999, ISSN: 8756-7938, DOI: 10.1002/btpr.2473
GORTE OLGA, HOLLENBACH REBECCA, PAPACHRISTOU IOANNIS, STEINWEG CHRISTIAN, SILVE AUDE, FREY WOLFGANG, SYLDATK CHRISTOPH, OCHSENREIT: "Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 8, 24 April 2020 (2020-04-24), pages 355, XP093066423, DOI: 10.3389/fbioe.2020.00355
GORTE OLGA, HOLLENBACH REBECCA, PAPACHRISTOU IOANNIS, STEINWEG CHRISTIAN, SILVE AUDE, FREY WOLFGANG, SYLDATK CHRISTOPH, OCHSENREIT: "Evaluation of Downstream Processing, Extraction, and Quantification Strategies for Single Cell Oil Produced by the Oleaginous Yeasts Saitozyma podzolica DSM 27192 and Apiotrichum porosum DSM 27194", FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, vol. 8, 24 April 2020 (2020-04-24), pages 355, XP093066423, DOI: 10.3389/fbioe.2020.00355
Attorney, Agent or Firm:
ARCO PATENT & TRADEMARK ATTORNEYS (JP)
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