METHOD FOR MEASURING CELL-MEDIATED IMMUNITY RESPONSE TO SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2 (SARS-COV-2)

Title:

METHOD FOR MEASURING CELL-MEDIATED IMMUNITY RESPONSE TO SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2 (SARS-COV-2)

Document Type and Number:

WIPO Patent Application WO/2023/167250

Kind Code:

A1

Abstract:

Established is a practical novel assay capable of measuring the cell-mediated immunity response of a subject to severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) with excellent simplicity, rapidity, and/or cost effectiveness, and high sensitivity. Provided is a method for measuring the cell-mediated immunity response of a subject to SARS‑CoV‑2. This method includes: (1) a step for preparing a sample containing white blood cells collected from a subject; (2) a step for adding a plurality of peptides to the sample and incubating the sample and the plurality of peptides in an aqueous medium; and (3) a step, following step (2), for measuring the level of the mRNA expression of a marker related to T lymphocyte function in white blood cells. The plurality of peptides correspond to a plurality of fragments of the amino acid sequence of the SARS‑CoV‑2 spike protein and cover at least 40% of the total length of the amino acid sequence of the SARS‑CoV‑2 spike protein.

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Inventors:

NAKATSURA TETSUYA (JP)
ARNAUD COUZINET (JP)
MURAKAMI TAKU (US)
SAITO TARO (JP)
KATAYAMA YUKI (JP)

Application Number:

PCT/JP2023/007658

Publication Date:

September 07, 2023

Filing Date:

March 01, 2023

Export Citation:

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Assignee:

NAT CANCER CT (JP)
RESONAC CORP (JP)

International Classes:

C12Q1/6844; C12N15/09; G01N33/50; G01N33/68

Domestic Patent References:

WO2022086444A1

2022-04-28

Foreign References:

JP2015529081A	2015-10-05
JP2018528974A	2018-10-04

Other References:

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LE BERT NINA, CLAPHAM HANNAH E., TAN ANTHONY T., CHIA WAN NI, THAM CHRISTINE Y.L., LIM JANE M., KUNASEGARAN KAMINI, TAN LINDA WEI : "Highly functional virus-specific cellular immune response in asymptomatic SARS-CoV-2 infection", JOURNAL OF EXPERIMENTAL MEDICINE, ROCKEFELLER UNIVERSITY PRESS, US, vol. 218, no. 5, 3 May 2021 (2021-05-03), US , XP055936764, ISSN: 0022-1007, DOI: 10.1084/jem.20202617
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