Title:
METHOD FOR MEASURING TOTAL MONOCYTES AND PERCENTAGE OF CD16-POSITIVE MONOCYTES
Document Type and Number:
WIPO Patent Application WO/2022/191293
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: a method for measuring the percentage of CD16-positive monocytes and the total monocytes in all nucleated cells in a sample; and a method for measuring the percentage of CD-16 positive monocytes among the total monocytes. The methods of the present invention are: a method for measuring the percentage of CD16-positive monocytes in all nucleated cells in a human sample, the method including analyzing a non-methylated state of one or more CpG positions in a marker region comprising at least one DNA sequence selected from the group consisting of SEQ ID NOS: 22, 25, 28, 31 and 34, wherein the percentage of CD16-positive monocytes in all nucleated cells in a human sample are calculated by using the percentage of non-methylated states of one or more CpG positions obtained via said analysis; and a method for measuring the percentage of CD16-positive monocytes in the total monocytes by using the aforementioned method.
Inventors:
NISHIOKA ERI (JP)
OGASAWARA HIDEAKI (JP)
IMAI TOSHIO (JP)
OGASAWARA HIDEAKI (JP)
IMAI TOSHIO (JP)
Application Number:
PCT/JP2022/010678
Publication Date:
September 15, 2022
Filing Date:
March 10, 2022
Export Citation:
Assignee:
EISAI R&D MAN CO LTD (JP)
International Classes:
C12N15/09; C12Q1/6869; C12Q1/6874
Foreign References:
| JP2018529346A | 2018-10-11 | |||
| JP2016515397A | 2016-05-30 | |||
| JP2021502806A | 2021-02-04 |
Other References:
ZAWADA ADAM M., SCHNEIDER JENNY S., MICHEL ANNE I., ROGACEV KYRILL S., HUMMEL BJÖRN, KREZDORN NICOLAS, MÜLLER SOEREN, ROTTER BJÖRN: "DNA methylation profiling reveals differences in the 3 human monocyte subsets and identifies uremia to induce DNA methylation changes during differentiation", EPIGENETICS, LANDES BIOSCIENCE, US, vol. 11, no. 4, 2 April 2016 (2016-04-02), US , pages 259 - 272, XP055965308, ISSN: 1559-2294, DOI: 10.1080/15592294.2016.1158363
ZHANG LU; HOFER THOMAS P.; ZAWADA ADAM M.; ROTTER BJöRN; KREZDORN NICOLAS; NOESSNER ELFRIEDE; DEVAUX YVAN; HEINE GUNNAR; ZIEG: "Epigenetics in non-classical monocytes support their pro-inflammatory gene expression", IMMUNOBIOLOGY, URBAN UND FISCHER VERLAG, DE, vol. 225, no. 3, 1 May 2020 (2020-05-01), DE , XP086169013, ISSN: 0171-2985, DOI: 10.1016/j.imbio.2020.151958
ZHANG LU; HOFER THOMAS P.; ZAWADA ADAM M.; ROTTER BJöRN; KREZDORN NICOLAS; NOESSNER ELFRIEDE; DEVAUX YVAN; HEINE GUNNAR; ZIEG: "Epigenetics in non-classical monocytes support their pro-inflammatory gene expression", IMMUNOBIOLOGY, URBAN UND FISCHER VERLAG, DE, vol. 225, no. 3, 1 May 2020 (2020-05-01), DE , XP086169013, ISSN: 0171-2985, DOI: 10.1016/j.imbio.2020.151958
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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