Title:
METHOD FOR MEASURING WATER SECRETION FUNCTION OF EPITHELIAL CELL
Document Type and Number:
WIPO Patent Application WO/2018/194036
Kind Code:
A1
Abstract:
The present invention enables easy evaluation of the water secretion function of an epithelial cell in a short time without involving staining with fluorescent pigment or the like. This method comprises: a preliminary culture step for seeding, in a culture vessel, organoids (700) established from epithelial cells and culturing the organoids in a gel (610) within each well (510) of the culture vessel for a prescribed period of time; a photographing step for taking, as a multi-pixel image, an image of each of the wells (510) containing the organoids (700); a detection step for detecting, as an object, a region, of the taken image, which is surrounded by edges and has a prescribed difference or larger in pixel intensity from the background thereof; a calculation step for calculating the area of said object inside the edges; a sorting step for sorting said object on the basis of whether or not the object is derived from the organoids; and a determination step for determining whether or not said object is to be analyzed, wherein a cell staining substance intended to stain the organoids (700) is not added in the photographing step.
Inventors:
WATANABE MAMORU (JP)
FUJII SATORU (JP)
OKAMOTO RYUICHI (JP)
FUJII SATORU (JP)
OKAMOTO RYUICHI (JP)
Application Number:
PCT/JP2018/015761
Publication Date:
October 25, 2018
Filing Date:
April 16, 2018
Export Citation:
Assignee:
UNIV NAT CORP TOKYO MEDICAL & DENTAL (JP)
SCREEN HOLDINGS CO LTD (JP)
SCREEN HOLDINGS CO LTD (JP)
International Classes:
C12Q1/02; C12M1/34
Domestic Patent References:
| WO2015177501A1 | 2015-11-26 | |||
| WO2013094365A1 | 2013-06-27 |
Foreign References:
| JP2016067280A | 2016-05-09 | |||
| JP2015192644A | 2015-11-05 | |||
| US20150078648A1 | 2015-03-19 |
Other References:
FUJII S. ET AL.: "PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells", SCI. REP., vol. 6, no. 36795, 9 November 2016 (2016-11-09), XP055560266, ISSN: 2045-2322, Retrieved from the Internet
Attorney, Agent or Firm:
NAKAJIMA, Jun et al. (JP)
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